• Immunofluorescence analysis of paraffin-embedded Human liver tissue using GLDC Rabbit polyclonal antibody (STJ11105408) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of Hep G2 cells using GLDC Rabbit polyclonal antibody (STJ11105408) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GLDC in paraffin-embedded human placenta using GLDC Rabbit polyclonal antibody (STJ11105408) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GLDC in paraffin-embedded human liver using GLDC Rabbit polyclonal antibody (STJ11105408) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GLDC in paraffin-embedded human kidney using GLDC Rabbit polyclonal antibody (STJ11105408) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using GLDC Rabbit polyclonal antibody (STJ11105408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using GLDC Rabbit polyclonal antibody (STJ11105408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GLDC antibody (580-749) (STJ11105408)

SKU:
STJ11105408

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Glycine Dehydrogenase (580-749) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLDC
Gene ID: 2731
Uniprot ID: GCSP_HUMAN
Immunogen Region: 580-749
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-749 of human GLDC (NP_000161.2).
Immunogen Sequence: HPFVPLDQAQGYQQLFRELE KDLCELTGYDQVCFQPNSGA QGEYAGLATIRAYLNQKGEG HRTVCLIPKSAHGTNPASAH MAGMKIQPVEVDKYGNIDAV HLKAMVDKHKENLAAIMITY PSTNGVFEENISDVCDLIHQ HGGQVYLDGANMNAQVGICR PGDFGSDVSH
Function The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor.CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
Protein Name Glycine Dehydrogenase
Decarboxylating - Mitochondrial
Glycine Cleavage System P Protein
Glycine Decarboxylase
Glycine Dehydrogenase
Aminomethyl-Transferring
Database Links Reactome: R-HSA-6783984
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glycine Dehydrogenase antibody
Anti-Decarboxylating - Mitochondrial antibody
Anti-Glycine Cleavage System P Protein antibody
Anti-Glycine Decarboxylase antibody
Anti-Glycine Dehydrogenase antibody
Anti-Aminomethyl-Transferring antibody
Anti-GLDC antibody
Anti-GCSP antibody

Information sourced from Uniprot.org

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