Anti-GGT5 antibody (388-587) (STJ116586)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GGT5 (388-587) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GGT5 |
| Gene ID | 2687 |
| Uniprot ID | GGT5_HUMAN |
| Immunogen Region | 388-587 |
| Immunogen Sequence | TSHVSVLGEDGSAVAATSTI NTPFGAMVYSPRTGIILNNE LLDLCERCPRGSGTTPSPAV SGDRVGGAPGRCWPPVPGER SPSSMVPSILINKAQGSKLV IGGAGGELIISAVAQAIMSK LWLGFDLRAAIAAPILHVNS KGCVEYEPNFSQEVQRGLQD RGQNQTQRPFFLNVVQAVSQ EGACVYAVSDLRKSGEAAGY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 388-587 of human GGT5 (NP_001093251.1). |
Additional Info
| Tissue Specificity | Expressed in follicular dendritic cells in lymphoid follicles (at protein level). |
| Post Translational Modifications | Cleaved by autocatalysis into a large and a small subunit. Glycosylated. |
| Function | Cleaves the gamma-glutamyl bond of extracellular glutathione tripeptide (gamma-Glu-Cys-Gly) and certain glutathione conjugates. Hydrolyzes glutathione releasing L-Glu and Cys-Gly dipeptide which is further metabolized to maintain extracellular cysteine levels but also to provide cysteine necessary for intracellular glutathione synthesis. Among glutathione-S-conjugates metabolizes leukotriene C4 (LTC4) and S-geranylgeranyl-glutathione (GGG), but is inactive toward gamma-glutamyl leucine. Converts extracellular LTC4 to LTD4 during acute inflammatory response. Acts as a negative regulator of GGG bioactivity. GGT5 (via GGG catabolism) and ABCC1 (via extracellular transport) establish GGG gradients within lymphoid tissues to position P2RY8-positive lymphocytes at germinal centers in lymphoid follicles and restrict their chemotactic transmigration from blood vessels to bone marrow parenchyma. The transpeptidation reaction, i.e. the transfer of gamma-glutamyl moiety to an acceptor molecule to yield a new gamma-glutamyl compound requires high concentration of dipeptide acceptor and is considered nonphysiological. |
| Protein Name | Glutathione Hydrolase 5 ProenzymeGamma-Glutamyl Transpeptidase-Related EnzymeGgt-RelGamma-Glutamyltransferase 5Ggt 5Gamma-Glutamyltransferase-Like Activity 1Gamma-Glutamyltranspeptidase 5Leukotriene-C4 Hydrolase Cleaved Into - Glutathione Hydrolase 5 Heavy Chain - Glutathione Hydrolase 5 Light Chain |
| Database Links | Reactome: R-HSA-174403Reactome: R-HSA-2142691Reactome: R-HSA-5423646Reactome: R-HSA-9664535Reactome: R-HSA-9753281 |
| Cellular Localisation | MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Glutathione Hydrolase 5 Proenzyme antibodyAnti-Gamma-Glutamyl Transpeptidase-Related Enzyme antibodyAnti-Ggt-Rel antibodyAnti-Gamma-Glutamyltransferase 5 antibodyAnti-Ggt 5 antibodyAnti-Gamma-Glutamyltransferase-Like Activity 1 antibodyAnti-Gamma-Glutamyltranspeptidase 5 antibodyAnti-Leukotriene-C4 Hydrolase Cleaved Into - Glutathione Hydrolase 5 Heavy Chain - Glutathione Hydrolase 5 Light Chain antibodyAnti-GGT5 antibodyAnti-GGTLA1 antibody |
Information sourced from Uniprot.org