• Immunofluorescence analysis of HeLa cells using Galactosidase alpha (Galactosidase alpha (GLA) ) antibody (STJ26175). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Galactosidase alpha (Galactosidase alpha (GLA) ) antibody (STJ26175) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-GLA antibody (150-429) (STJ26175)

SKU:
STJ26175

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLA (150-429) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLA
Gene ID: 2717
Uniprot ID: AGAL_HUMAN
Immunogen Region: 150-429
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human Galactosidase alpha (Galactosidase alpha (GLA) ) (NP_000160.1).
Immunogen Sequence: GYYDIDAQTFADWGVDLLKF DGCYCDSLENLADGYKHMSL ALNRTGRSIVYSCEWPLYMW PFQKPNYTEIRQYCNHWRNF ADIDDSWKSIKSILDWTSFN QERIVDVAGPGGWNDPDMLV IGNFGLSWNQQVTQMALWAI MAAPLFMSNDLRHISPQAKA LLQDKDVIAINQDPLGKQGY QLRQGDNFEVWERPLSGLAW AVAMINRQEIGGPRSYTIAV ASLGKGVACNPACFITQLL
Function Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
Protein Name Alpha-Galactosidase A
Alpha-D-Galactosidase A
Alpha-D-Galactoside Galactohydrolase
Galactosylgalactosylglucosylceramidase Gla
Melibiase
Agalsidase
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Alpha-Galactosidase A antibody
Anti-Alpha-D-Galactosidase A antibody
Anti-Alpha-D-Galactoside Galactohydrolase antibody
Anti-Galactosylgalactosylglucosylceramidase Gla antibody
Anti-Melibiase antibody
Anti-Agalsidase antibody
Anti-GLA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance