• Western blot analysis of extracts of various cell lines, using GLDC antibody (STJ111974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunoprecipitation analysis of 200 Mu g extracts of DU145 cells using 3 Mu g GLDC antibody (STJ111974). Western blot was performed from the immunoprecipitate using GLDC antibody (STJ111974) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 200ug extracts of DU145 cells using 3ug GLDC antibody (STJ111974). Western blot was performed from the immunoprecipitate using GLDC antibody (STJ111974) at a dilition of 1:1000.
  • Western blot analysis of various lysates using GLDC Rabbit polyclonal antibody (STJ111974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GLDC antibody (36-290) (STJ111974)

SKU:
STJ111974

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLDC (36-290) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLDC
Gene ID: 2731
Uniprot ID: GCSP_HUMAN
Immunogen Region: 36-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2).
Immunogen Sequence: DSSSGGGDSAAAGASRLLER LLPRHDDFARRHIGPGDKDQ REMLQTLGLASIDELIEKTV PANIRLKRPLKMEDPVCENE ILATLHAISSKNQIWRSYIG MGYYNCSVPQTILRNLLENS GWITQYTPYQPEVSQGRLES LLNYQTMVCDITGLDMANAS LLDEGTAAAEALQLCYRHNK RRKFLVDPRCHPQTIAVVQT RAKYTGVLTELKLPCEMDFS GKDVSGVLFQYPDTEGKVE
Function The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor.CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
Protein Name Glycine Dehydrogenase
Decarboxylating - Mitochondrial
Glycine Cleavage System P Protein
Glycine Decarboxylase
Glycine Dehydrogenase
Aminomethyl-Transferring
Database Links Reactome: R-HSA-6783984
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glycine Dehydrogenase antibody
Anti-Decarboxylating - Mitochondrial antibody
Anti-Glycine Cleavage System P Protein antibody
Anti-Glycine Decarboxylase antibody
Anti-Glycine Dehydrogenase antibody
Anti-Aminomethyl-Transferring antibody
Anti-GLDC antibody
Anti-GCSP antibody

Information sourced from Uniprot.org

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