Western blot analysis of various lysates using GLDC Rabbit pAb (STJ111974) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GLDC antibody (36-290) (STJ111974)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111974
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General Information

Short DescriptionRabbit polyclonal GLDC (36-290) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/IP/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:100
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGLDC
Gene ID2731
Uniprot IDGCSP_HUMAN
Immunogen Region36-290
Immunogen SequenceDSSSGGGDSAAAGASRLLER LLPRHDDFARRHIGPGDKDQ REMLQTLGLASIDELIEKTV PANIRLKRPLKMEDPVCENE ILATLHAISSKNQIWRSYIG MGYYNCSVPQTILRNLLENS GWITQYTPYQPEVSQGRLES LLNYQTMVCDITGLDMANAS LLDEGTAAAEALQLCYRHNK RRKFLVDPRCHPQTIAVVQT RAKYTGVLTELKLPCEMDFS GKDVSGVLFQYPDTEGKVE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2).

Additional Info

Function The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor.CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
Protein Name Glycine Dehydrogenase
Decarboxylating - Mitochondrial
Glycine Cleavage System P Protein
Glycine Decarboxylase
Glycine Dehydrogenase
Aminomethyl-Transferring
Database Links Reactome: R-HSA-6783984
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glycine Dehydrogenase antibody
Anti-Decarboxylating - Mitochondrial antibody
Anti-Glycine Cleavage System P Protein antibody
Anti-Glycine Decarboxylase antibody
Anti-Glycine Dehydrogenase antibody
Anti-Aminomethyl-Transferring antibody
Anti-GLDC antibody
Anti-GCSP antibody

Information sourced from Uniprot.org

Citations

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