Anti-GID8 antibody (115-165 aa) (STJ198160)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human GID8 at the amino acid range 115-165
General Information
| Short Description | Rabbit polyclonal anti-Glucose-induced degradation protein 8 homolog (115-165 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GID8 |
| Gene ID | 54994 |
| Uniprot ID | GID8_HUMAN |
| Immunogen | Synthesized peptide derived from the human GID8 at the amino acid range 115-165 |
| Immunogen Region | 115-165 aa |
| Specificity | This antibody detects endogenous levels of human GID8 |
Additional Info
| Post Translational Modifications | Polyubiquitinated through 'Lys-48'-polyubiquitin chains, leading to proteasomal degradation in the absence of Wnt stimulation. |
| Function | Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation. |
| Protein Name | Glucose-Induced Degradation Protein 8 HomologTwo Hybrid-Associated Protein 1 With RanbpmTwa1 |
| Database Links | Reactome: R-HSA-9861718 |
| Cellular Localisation | CytoplasmNucleusLocalizes In The Cytoplasm In The Absence Of Wnt Stimulation And In The Nucleus In The Presence Of Wnt Stimulation |
| Alternative Antibody Names | Anti-Glucose-Induced Degradation Protein 8 Homolog antibodyAnti-Two Hybrid-Associated Protein 1 With Ranbpm antibodyAnti-Twa1 antibodyAnti-GID8 antibodyAnti-C20orf11 antibodyAnti-TWA1 antibody |
Information sourced from Uniprot.org