Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis using HDAC3 monoclonal antibody against THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3, HeLa cell lysate.

Anti-HDAC3 antibody (STJ98496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Rabbit

€180.00 - €435.00

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Western blot detection of HDAC3 in THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3 and Hela cell lysates using HDAC3 mouse mAb (1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa.

Anti-HDAC3 antibody [3G3-H6-H10] (STJ99157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€180.00 - €435.00

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Western blot analysis of various lysates, using HDAC4 antibody (STJ11101423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HDAC4 antibody (1-100) [S3MR] (STJ11101423)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Immunohistochemistry analysis of HDAC4 in paraffin-embedded mouse brain using HDAC4 Rabbit polyclonal antibody (STJ113153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC4 antibody (451-650) (STJ113153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates, using HDAC4 Rabbit polyclonal antibody (STJ29788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC4 antibody (530-630) (STJ29788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various cells using HDAC4 Polyclonal Antibody diluted at 1：2000

Anti-HDAC4 antibody (598-647 aa) (STJ93480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis using HDAC4 monoclonal antibody against Jurkat, K562 cell lysate.

Anti-HDAC4 antibody (N-Term) (STJ98497)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€180.00 - €435.00

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Western blot detection of HDAC4 in Jurkat, Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa.

Anti-HDAC4 antibody [4A3-H7-C6] (STJ99035)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

€180.00 - €435.00

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Immunohistochemistry analysis of HDAC7 in paraffin-embedded mouse spinal cord using HDAC7 Rabbit polyclonal antibody (STJ11100893) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC7 antibody (1-100) (STJ11100893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using HDAC7 Polyclonal Antibody diluted at 1：1000

Anti-HDAC7 antibody (121-170 aa) (STJ93484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using HDAC7 Rabbit monoclonal antibody (STJ11101421) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HDAC7 antibody (24-198) [S1MR] (STJ11101421)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of extracts of various cell lines, using HDAC7 antibody (STJ29424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HDAC7 antibody (400-500) (STJ29424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using Histone deacetylase 7a Polyclonal Antibody

Anti-HDAC7 antibody (901-950 aa) (STJ93513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-HERC2 antibody (646-696 aa) (STJ197613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Hrs Polyclonal Antibody diluted at 1：500

Anti-HGS antibody (301-350 aa) (STJ93593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using HGS antibody (STJ28094) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HGS antibody (548-777) (STJ28094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-HGS antibody (C-Term) (STJ13101216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat Mouse

€414.38

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Anti-HIC1 antibody (STJA0007757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry of mouse lung with Anti-HIF-1-Alpha isoform 1 + 2 primary antibody (STJ501335) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) (STJ501335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) {Biotin} (STJ501338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) {FITC} (STJ501339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemistry of mouse lung with Anti-HIF-1-Alpha isoform 1 primary antibody (STJ501334) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-HIF-1-Alpha isoform 1 antibody (790-826) (STJ501334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-HIF-1-Alpha isoform 1 antibody (790-826) {Biotin} (STJ501336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-HIF-1-Alpha isoform 1 antibody (790-826) {FITC} (STJ501337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-HIF1 Alpha antibody (552-826 aa) (STJ11106258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from 1) Hela, 2) 293, 3)mouse braincells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-HIF1A antibody (328-377 aa) (STJ93498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-HIF1a antibody at 2500 dilution; HT1080 cells in the presence of CoCl2; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-HIF1A antibody (470 to 590aa) (STJ140124)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€169.38 - €493.13

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Western blot analysis of lysates from HepG2 cells, using HIF1 Alpha Rabbit polyclonal antibody (STJ29997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HIF1A antibody (600-826) (STJ29997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from HeLa cells, using HIF1 Alpha Rabbit pAb (STJ113863) at 1:1000 dilution. HeLa cells were treated by Cobalt chloride (0. 1 mM) at 37 °C for 4 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-HIF1A antibody (670-826) (STJ113863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-HIF1A antibody (780aa C-Term) (STJ140040)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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Western blot analysis using HIF-1 Alpha monoclonal antibody against Cos7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.

Anti-HIF1A antibody [1A3] (STJ98135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€180.00 - €435.00

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Western blot analysis of 1) HepG2 Cell, 2) HepG2 Treated with CoCl2 cell lysate using HIF-1α mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HIF1A antibody [Mix] (STJ196669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€217.50 - €315.00

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Immunofluorescence analysis of C6 cells using HIF3A Rabbit polyclonal antibody (STJ11101790) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HIF3A antibody (1-130) (STJ11101790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using HIPK2 Rabbit mAb (STJ11102490) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HIPK2 antibody (100-200) [S0MR] (STJ11102490)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Anti-HIPK2 antibody (1150-1198) (STJ501343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-HIPK2 antibody (1150-1198) {Biotin} (STJ501344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-HIPK2 antibody (1150-1198) {FITC} (STJ501345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-HIPK2 antibody (STJ191972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-HIST1H2AH antibody (STJA0004677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

€175.63 - €519.38

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Anti-HIST1H2AH antibody (STJA0004678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

€175.63 - €519.38

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Western blot analysis of extracts of various cell lines, using Histone H2A. Z rabbit monoclonal antibody (STJ11102114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Histone H2A antibody (1-100) [S4MR] (STJ11102114)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

€183.13 - €803.13

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Western blot analysis of lysates from wild type (WT) and Histone H2A. Z knockout (KO) 293T cells, using [KO Validated] Histone H2A. Z Rabbit polyclonal antibody (STJ114316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Histone H2A antibody (1-128) (STJ114316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Histone H2A antibody (1-128) (STJ28697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€140.63 - €655.63

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H2A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Histone H2A antibody (Internal) (STJ96865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Histone H2AX antibody (1-143 aa) (STJ11106040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

€140.63 - €655.63

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3. 3 rabbit monoclonal antibody (STJ11102163). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Histone H3 antibody (57-136) [S3MR] (STJ11102163)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

€183.13 - €803.13

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