Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of various lysates using SCFD1 Rabbit pAb (STJ111440) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-SCFD1 antibody (373-642) (STJ111440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using SCFD1 Polyclonal Antibody

Anti-SCFD1 antibody (533-582 aa) (STJ95586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using SCG2 antibody (STJ28508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SCG2 antibody (30-320) (STJ28508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Anti-SCG2 antibody (STJA0007696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SCG3 antibody (199-468 aa) (STJ11106075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using SCG3 antibody (STJ110109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SCG3 antibody (199-468) (STJ110109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of mouse heart, mouse kidney cells using Sg III Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SCG3 antibody (21-70 aa) (STJ96507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-SCG3 antibody [SAA1746] (STJA0020174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Anti-SDC2 antibody (19-144 aa) (STJ11106066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of SDC2 in paraffin-embedded human esophagus using SDC2 Rabbit polyclonal antibody (STJ25458) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SDC2 antibody (19-144) (STJ25458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SDC2 Antibody. The lane on the right is blocked with the SDC2 peptide.

Anti-SDC2 antibody (81-130 aa) (STJ95855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using SEC11A Rabbit polyclonal antibody (STJ112570) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SEC11A antibody (37-145) (STJ112570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SEC11A antibody (STJA0007759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using SEC13 Rabbit polyclonal antibody (STJ113218) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SEC13 antibody (1-322) (STJ113218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SEC13 antibody (STJA0007212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SEC16A Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-SEC16A antibody (1013-1062 aa) (STJ95593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-SEC22A antibody (STJ13100338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-SEC22A antibody (STJ13102047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-SEC22B antibody (2-50 aa) (STJ13101991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-SEC22B antibody (2-50 aa) (STJ13101995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of JK using SEC22B antibody.. Secondary antibody was diluted at 1:20000

Anti-SEC22B antibody (Internal) (STJ97633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SEC22B antibody (STJ13102012)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-SEC22B antibody (STJ13102013)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SEC22C antibody (130-180 aa) (STJ195371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-SEC22C antibody (C-Term) (STJ13102048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Anti-SEC22C antibody (C-Term) (STJ13102049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Western blot analysis of various lysates using Sec23A Rabbit polyclonal antibody (STJ113995) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SEC23A antibody (1-150) (STJ113995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Hep G2 cells, using Sec23AA Rabbit mAb (STJ11102342) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-SEC23A antibody (300-400) [S2MR] (STJ11102342)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SEC23A antibody (352-402 aa) (STJ193768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry of baboon Bladder with Anti-SEC23A primary antibody (STJ502891) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SEC23A antibody (700-765) (STJ502891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey

£427.50

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Anti-SEC23A antibody (700-765) {Biotin} (STJ502892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey

£528.50

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Anti-SEC23A antibody (700-765) {FITC} (STJ502893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey

£528.50

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Immunofluorescence analysis of L929 cells using SEC23IP Rabbit polyclonal antibody (STJ117593) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SEC23IP antibody (600-940) (STJ117593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SEC23IP antibody (823-873) (STJA0006346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-SEC24A antibody (STJA0007053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-SEC24B antibody (215-250 aa) (STJ13102268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various cell lines, using SEC24C antibody (STJ112693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SEC24C antibody (915-1094) (STJ112693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from NIH/3T3 cells, using SEC24D Rabbit monoclonal antibody (STJ11104196) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEC24D antibody (1-100) [S4196RM] (STJ11104196)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of L929 cells using SEC24D Rabbit polyclonal antibody (STJ118531) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SEC24D antibody (830-1000) (STJ118531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-SEC24D Antibody (STJ502903) on rat hippocampal membranes (A) and Hu cells (B). Antibody at 1:500 dilution in buffer.

Anti-SEC24D antibody (860-920) (STJ502903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IHC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SEC24D antibody (860-920) {Biotin} (STJ502904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IHC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SEC24D antibody (STJA0009260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Anti-SEC31A antibody (STJA0006984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Sec61A Rabbit monoclonal antibody (STJ11103140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Sec61A antibody (350-450) [S0MR] (STJ11103140)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of A-549 cells, using SEC61A1 Antibody (STJ113219) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SEC61A1 antibody (310-420) (STJ113219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SEC61A1 Rabbit polyclonal antibody (STJ113260) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SEC61A1 antibody (310-420) (STJ113260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SEC61A1 antibody (333-383 aa) (STJ194167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SEC61A2 antibody (318-368 aa) (STJ194440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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