• Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-RBBP5 mouse mAb (dilution 1:100). DAPI was used to stain nucleus (blue).
  • Western blot detection of RBBP5 in C2C12, THP-1, PC12 and 3T3 cell lysates using RBBP5 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Anti-RBBP5 antibody [1C9-1C9-H10] (STJ99267)

SKU:
STJ99267

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Retinoblastoma-binding protein 5 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 1C9-1C9-H10
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:100
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBBP5
Gene ID: 5929
Uniprot ID: RBBP5_HUMAN
Specificity: This antibody detects endogenous levels of RBBP5 and does not cross-react with related proteins.
Immunogen: Purified recombinant human RBBP5 protein fragments expressed in E.coli.
Function In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Protein Name Retinoblastoma-Binding Protein 5
Rbbp-5
Retinoblastoma-Binding Protein Rbq-3
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-3214841
Reactome: R-HSA-3769402
Reactome: R-HSA-5617472
Reactome: R-HSA-8936459
Reactome: R-HSA-8951664
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Retinoblastoma-Binding Protein 5 antibody
Anti-Rbbp-5 antibody
Anti-Retinoblastoma-Binding Protein Rbq-3 antibody
Anti-RBBP5 antibody
Anti-RBQ3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance