• Western blot analysis of extracts of various cell lines, using RanGAP1 rabbit monoclonal antibody (STJ11102370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of U-2 OS cells using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat testis using RanGAP1 rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human placenta using RanGAP1 rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using RanGAP1 rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RanGAP1 in paraffin-embedded mouse kidney tissue using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using RanGAP1 rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RanGAP1 in paraffin-embedded rat liver tissue using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using RanGAP1 rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RanGAP1 in paraffin-embedded human colon carcinoma tissue using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using RanGAP1 rabbit monoclonal antibody (STJ11102370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RanGAP1 in paraffin-embedded mouse brain tissue using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of RanGAP1 in paraffin-embedded rat testis tissue using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using RanGAP1 Rabbit monoclonal antibody (STJ11102370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RANGAP1 antibody (1-100) [S0MR] (STJ11102370)

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STJ11102370

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RanGAP1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RANGAP1
Gene ID: 5905
Uniprot ID: RAGP1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RanGAP1 (P46060).
Immunogen Sequence: MASEDIAKLAETLAKTQVAG GQLSFKGKSLKLNTAEDAKD VIKEIEDFDSLEALRLEGNT VGVEAARVIAKALEKKSELK RCHWSDMFTGRLRTEIPPAL
Tissue Specificity Highly expressed in brain, thymus and testis.
Post Translational Modifications Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBE2I. Desumoylated by HINT1.
Function GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export.
Protein Name Ran Gtpase-Activating Protein 1
Rangap1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-165054
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-4615885
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9615933
Reactome: R-HSA-9648025
Reactome: R-HSA-9793242
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleus Envelope
Chromosome
Centromere
Kinetochore
Cytoskeleton
Spindle
Cytoplasmic During Interphase
Detected At The Nuclear Envelope During Interphase
Targeted To The Nuclear Pores After Sumoylation
During Mitosis
Associates With Mitotic Spindles
But Is Essentially Not Detected At The Spindle Poles
Association With Kinetochores Appears Soon After Nuclear Envelope Breakdown And Persists Until Late Anaphase
Mitotic Location Also Requires Sumoylation
Alternative Antibody Names Anti-Ran Gtpase-Activating Protein 1 antibody
Anti-Rangap1 antibody
Anti-RANGAP1 antibody
Anti-KIAA1835 antibody
Anti-SD antibody

Information sourced from Uniprot.org

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