Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of extracts of mouse brain, using RGS7 antibody (STJ28803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RGS7 antibody (1-300) (STJ28803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Jurkat cells, using RGS7 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-RGS7 antibody (155-204 aa) (STJ95441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-RGS9 antibody (STJA0008372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of HUVEC cells, using RhoA antibody (STJ11101643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RHOA antibody (1-100) [S3MR] (STJ11101643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Immunofluorescence analysis of U2OS cells using RhoA antibody (STJ118088) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RHOA antibody (1-193) (STJ118088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-RhoA antibody (100-150 aa) (STJ13100298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

£331.50

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Anti-RhoA antibody (100-150 aa) (STJ13100302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

£331.50

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Immunofluorescence analysis of Hela cell. 1, Rho A Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-RHOA antibody (144-193 aa) (STJ95442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of RhoA in paraffin-embedded human placenta using RhoA Rabbit polyclonal antibody (STJ115882) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-RHOA antibody (94-193) (STJ115882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using RhoA antibody (STJ25351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RHOA antibody (94-193) (STJ25351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of Hela Cell lysate using RhoA mouse Monoclonal monoclonal antibody diluted at 1:1,000.

Anti-RHOA antibody [4E12] (STJ196673)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-RhoA antibody [PT0088R] (STJA0039751)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit polyclonal antibody (STJ11104873) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RHOT1 antibody (310-590) (STJ11104873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-RHOT1 antibody (310-590) (STJ28401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-RHOT1 antibody (50-100 aa) (STJ13100187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-RHOT1/MIRO1 antibody [SRM14536] (STJ11109266)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of RAW264. 7, using Rig-I/DDX58 antibody (STJ11104856) at 1:2000 dilution. Raw264. 7 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RIGI antibody (241-455) [S4856RM] (STJ11104856)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts from rat liver, using Rig-I/DDX58 Rabbit monoclonal antibody (STJ11105039) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RIGI antibody (241-455) [S5039RM] (STJ11105039)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Anti-RIGI antibody (STJ192214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using Rig-I/DDX58 antibody (STJ11105683) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RIGI antibody [S5683RM] (STJ11105683)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-RING1 antibody (STJA0009464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g RING1A antibody (STJ11103112). Western blot was performed from the immunoprecipitate using RING1A antibody (STJ11103112) at a dilution of 1:1000.

Anti-RING1A antibody (100-200) [S2MR] (STJ11103112)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of RING1A in paraffin-embedded mouse stomach using RING1A Rabbit polyclonal antibody (STJ11103202) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-RING1A antibody (207-406) (STJ11103202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-RING1B/RNF2 antibody [SRM13426] (STJ11108156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RIOX1 antibody (533-583 aa) (STJ195036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-RIOX1/C14ORF169 IP antibody [SAA0194] (STJA0018709)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Anti-RIOX2 antibody (STJ191782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using RIOX2/RIOX2/MINA53 Rabbit monoclonal antibody (STJ11103150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RIOX2/MINA53 antibody (350-450) [S0MR] (STJ11103150)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of NIH/3T3 cells using RIPK1/RIP Rabbit polyclonal antibody (STJ29550) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RIPK1 antibody (100-200) (STJ29550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g RIPK1/RIP Rabbit monoclonal antibody (STJ11101710). Western blot was performed from the immunoprecipitate using RIPK1/RIP Rabbit monoclonal antibody (STJ11101710) at a dilution of 1:500.

Anti-RIPK1 antibody (289-490) [S0MR] (STJ11101710)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-RIPK1 antibody (620-670) (STJ502798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-RIPK1 antibody (620-670) {Biotin} (STJ502799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-RIPK1 antibody (620-670) {FITC} (STJ502800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-RIPK1 antibody (STJ191954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-RIPK1/RIP antibody (STJ11108302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of extracts of various cell lines, using RIPK2 antibody (STJ25358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RIPK2 antibody (1-540) (STJ25358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 lysis using RIP2 antibody. Antibody was diluted at 1:500

Anti-RIPK2 antibody (146-195 aa) (STJ95512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-RIPK2 antibody (410-460) (STJ502801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Pig/Monkey

£427.50

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Anti-RIPK2 antibody (410-460) {Biotin} (STJ502802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Pig/Monkey

£528.50

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Anti-RIPK2 antibody (410-460) {FITC} (STJ502803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Pig/Monkey

£528.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RNF103 antibody (243-293 aa) (STJ195916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-RNF123 antibody (STJ190791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-RNF128 antibody (STJA0008289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using RNF135 Rabbit pAb (STJ11107541) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-RNF135 antibody (121-309 aa) (STJ11107541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RNF135 antibody (81-130 aa) (STJ192588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RNF139 antibody (10-60 aa) (STJ196033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-RNF139 antibody (500-550 aa) (STJ13101959)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of various cells using UBCE7IP4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-RNF144A antibody (151-200 aa) (STJ96161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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