• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PSMB2 antibody (STJ27436). Western blot was performed from the immunoprecipitate using PSMB2 antibody (STJ27436) at a dilution of 1:1000.
  • Immunohistochemistry analysis of PSMB2 in paraffin-embedded human esophageal cancer using PSMB2 Rabbit polyclonal antibody (STJ27436) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PSMB2 Rabbit polyclonal antibody (STJ27436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using PSMB2 Rabbit polyclonal antibody (STJ27436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSMB2 antibody (1-201) (STJ27436)

SKU:
STJ27436

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSMB2 (1-201) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSMB2
Gene ID: 5690
Uniprot ID: PSB2_HUMAN
Immunogen Region: 1-201
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human PSMB2 (NP_002785.1).
Immunogen Sequence: MEYLIGIQGPDYVLVASDRV AASNIVQMKDDHDKMFKMSE KILLLCVGEAGDTVQFAEYI QKNVQLYKMRNGYELSPTAA ANFTRRNLADCLRSRTPYHV NLLLAGYDEHEGPALYYMDY LAALAKAPFAAHGYGAFLTL SILDRYYTPTISRERAVELL RKCLEELQKRFILNLPTFSV RIIDKNGIHDLDNISFPKQG S
Function Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Protein Name Proteasome Subunit Beta Type-2
Macropain Subunit C7-I
Multicatalytic Endopeptidase Complex Subunit C7-I
Proteasome Component C7-I
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1234176
Reactome: R-HSA-1236974
Reactome: R-HSA-1236978
Reactome: R-HSA-174084
Reactome: R-HSA-174113
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-180534
Reactome: R-HSA-180585
Reactome: R-HSA-187577
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-211733
Reactome: R-HSA-2467813
Reactome: R-HSA-2871837
Reactome: R-HSA-349425
Reactome: R-HSA-350562
Reactome: R-HSA-382556
Reactome: R-HSA-450408
Reactome: R-HSA-4608870
Reactome: R-HSA-4641257
Reactome: R-HSA-4641258
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5658442
Reactome: R-HSA-5668541
Reactome: R-HSA-5676590
Reactome: R-HSA-5678895
Reactome: R-HSA-5687128
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69481
Reactome: R-HSA-69601
Reactome: R-HSA-75815
Reactome: R-HSA-8852276
Reactome: R-HSA-8854050
Reactome: R-HSA-8939236
Reactome: R-HSA-8939902
Reactome: R-HSA-8941858
Reactome: R-HSA-8948751
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9020702
Reactome: R-HSA-9604323
Reactome: R-HSA-9755511
Reactome: R-HSA-9762114
Reactome: R-HSA-9824272
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Translocated From The Cytoplasm Into The Nucleus Following Interaction With Akirin2
Which Bridges The Proteasome With The Nuclear Import Receptor Ipo9
Alternative Antibody Names Anti-Proteasome Subunit Beta Type-2 antibody
Anti-Macropain Subunit C7-I antibody
Anti-Multicatalytic Endopeptidase Complex Subunit C7-I antibody
Anti-Proteasome Component C7-I antibody
Anti-PSMB2 antibody

Information sourced from Uniprot.org

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