Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of Jurkat, using Caspase-10 Rabbit polyclonal antibody (STJ22895) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CASP10 antibody (220-521) (STJ22895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of extracts from Jurkat, K562 cells, using Caspase-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CASP10 antibody (Internal) (STJ96459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using Caspase-2 Polyclonal Antibody

Anti-CASP2 antibody (123-172 aa) (STJ92020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using CASP2 Polyclonal Antibody (STJ28291) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CASP2 antibody (153-452) (STJ28291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using Caspase-2 polyclonal antibody (STJ11102181) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP2 antibody (334-452) [S1MR] (STJ11102181)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of extracts of Jurkat cells, using Active Caspase-3 Rabbit monoclonal antibody (STJ112894) at 1:500 dilution. Jurkat cells were treated by Staurosporine (1 Mu M) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP3 antibody (1-100) [S4MR] (STJ112894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Caspase 3 antibody western blot K562 HeLa STJA0010811

Anti-CASP3 antibody (1-20 aa) [R06-1B2] (STJA0010811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Western blot analysis of various cells using Caspase-3 Polyclonal Antibody diluted at 1：1000

Anti-CASP3 antibody (116-165 aa) (STJ92021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Fish

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells using Caspase-3 p12 antibody (STJ11101753). HeLa cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (176-277) [S3MR] (STJ11101753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-CASP3 antibody (20-100) [5H10] (STJ97730)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IF-P

Reactivity :

Human/Rat/Mouse

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Western blot analysis of lysates from wild type (WT) and active + pro Caspase-3 knockout (KO) 293T cells, using [KO Validated] active + pro Caspase-3 Rabbit monoclonal antibody (STJ11101749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP3 antibody (29-175) [S9MR] (STJ11101749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$198.03 - $853.53

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Western blot analysis of various lysates using Caspase-3 Mouse monoclonal antibody (STJ119900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP3 antibody (55-160) [S0MM] (STJ119900)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of various lysates using [KO Validated] Caspase-3 rabbit polyclonal antibody (STJ22900) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CASP3 antibody (55-277) (STJ22900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-CASP3 antibody (6-105) (STJ113073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Caspase 3 antibody immunocytochemistry 293 cells STJA0010817

Anti-CASP3 antibody (75-94 aa) [R02-7C2] (STJA0010817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-CASP3 antibody (N-Term) [M377] (STJA0005295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

$440.91

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Anti-CASP3 antibody [31A1067] (STJA0005296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$440.91

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Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP3 antibody [5E1] (STJ97448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IP/IHC

Reactivity :

Human/Mouse/Rat/Chicken

$67.62 - $360.18

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Anti-CASP3 nanobody [VHH1] (STJN000623)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$269.79 - $1,820.91

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Anti-CASP3 nanobody [VHH2] (STJN000624)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$269.79 - $1,820.91

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Anti-CASP3/Caspase-3 nanobody [SAA1223] (STJN000220)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF

Reactivity :

Human

$269.79 - $1,820.91

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Immunohistochemistry analysis of Caspase-4 antibody in paraffin-embedded human lung carcinoma tissue.

Anti-CASP4 antibody (75-124 aa) (STJ92022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using Caspase-4 Rabbit polyclonal antibody (STJ11100597) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASP4 antibody (81-270) (STJ11100597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-CASP8 antibody (200-300) [S2MR] (STJ11101692)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ29787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Casp8 antibody (376-479) (STJ29787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-CASP8 antibody (C-Term) [M382] (STJA0005298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$440.91

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57 Yang B, Wang L, Luo X, et al. SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis[J]. International journal of oncology, 2015, 46 (2) : 649-656.

Anti-CASP8 antibody (STJ193171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat/Pig/Chicken

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP8 antibody [2G12] (STJ97394)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ110935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP9 antibody (139-416) (STJ110935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using Caspase-9 Rabbit pAb (STJ22908) at 1:300 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CASP9 antibody (139-416) (STJ22908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using Caspase-9 Polyclonal Antibody diluted at 1：500

Anti-CASP9 antibody (91-140 aa) (STJ92027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP9 antibody [3-20] (STJ96979)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/Chicken

$67.62 - $360.18

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Anti-CASP9/Caspase 9 antibody (Lys100-Thr270) [1A127] (STJA0021960)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$272.55 - $380.19

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Caspase1 antibody western blot HeLa lysates STJA0034107

Anti-Caspase 1 antibody [R05-7H1] (STJA0034107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$184.23 - $388.47

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Caspase1 antibody western blot HeLa lysates STJA0035924

Anti-Caspase 1 antibody [R07-7Q3] (STJA0035924)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

$184.23 - $388.47

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Caspase 10 antibody western blot K562 lysates STJA0034108

Anti-Caspase 10 antibody [R03-2J3] (STJA0034108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$184.23 - $388.47

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Caspase 10 antibody western blot HeLa lysates STJA0036080

Anti-Caspase 10 antibody [R03-3B2] (STJA0036080)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse

$184.23 - $388.47

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Caspase 3 antibody immunohistochemistry human skin tissue STJA0032895

Anti-Caspase 3 antibody [1B10-10E2-6A5] (STJA0032895)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Caspase 3 antibody immunohistochemistry human skin tissue STJA0032894

Anti-Caspase 3 antibody [5H3-3H7-7G2] (STJA0032894)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Caspase 3 antibody western blot K562 rat brain C6 3T3 HeLa STJA0033942

Anti-Caspase 3 antibody [R08-6F2] (STJA0033942)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Caspase 5 antibody western blot HeLa cells STJA0036074

Anti-Caspase 5 antibody [R06-1D5] (STJA0036074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-Caspase 8 antibody (STJA0033160)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$258.75 - $388.47

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Anti-Caspase 8 antibody [R02-0D7] (STJA0034113)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Hamster

$193.89 - $425.73

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Caspase 8 antibody western blot HeLa cells STJA0036081

Anti-Caspase 8 antibody [R06-3K2] (STJA0036081)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Caspase9 antibody western blot A549 MCF-7 Jurkat K562 HeLa STJA0032455

Anti-Caspase 9 antibody [1D1-F2-E9] (STJA0032455)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$184.23 - $388.47

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Anti-Caspase 9 antibody [R01-2C2] (STJA0035143)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$193.89 - $425.73

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Anti-Caspase 9 antibody [R05-4I8] (STJA0036098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

$193.89 - $425.73

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Anti-Caspase 9 antibody [R09-4C4] (STJA0033883)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse

$193.89 - $425.73

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