Immune System | Research Area | St John's Laboratory

Anti-Phospho-LIMK1-Ser-323 antibody (STJA0005461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western Blot analysis of 293T using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-LIMK1-Ser310 antibody (STJA0009991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-LIMK1-T508 Rabbit pAb (STJ110833).

Anti-Phospho-LIMK1-T508 antibody (STJ110833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-LIMK1-Thr-508 antibody (STJA0005462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of various cells using Phospho-LIMK-1 (T508) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-LIMK1-Thr508 antibody (471-520 aa) (STJ90321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Lyn antibody (425-525) (STJ502546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-Lyn antibody (425-525) {Biotin} (STJ502547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Lyn antibody (425-525) {FITC} (STJ502548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-LYN-Tyr507 antibody (STJ196350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Lyn (Y508) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-LYN-Tyr508 antibody (463-512 aa) (STJ90764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y396 Rabbit mAb (STJ11102620) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-LYN-Y396 antibody [S0MR] (STJ11102620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-Lyn-Y507 antibody (STJ11107669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-pS221 Rabbit pAb (STJ22232) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-pS221 antibody (STJ22232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-S217 Rabbit pAb (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K1-S217 antibody (STJ22230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (STJ22231) at 1:1000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAP2K1-S298 antibody (STJ22231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (STJ11102602) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of HEPG2-UV using p-MEK-1 (S298) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP2K1-Ser298 antibody (264-313 aa) (STJ90489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (STJ22233) at 1:2000 dilution or MEK1 antibody (STJ114560). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by ATP (5 mM) at 30 °C for 1 hour. PC-12 cells were treated by Nocodazole (1 Mu g/mL) at 37 °C for 16 hours or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-MAP2K1-T286 antibody (STJ22233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (STJ11102603) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K1-Thr286 antibody (252-301 aa) (STJ90854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP2K1-Thr292 antibody (261-310 aa) (STJ90326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of recombinant wild type MEK 1 (WT) and mutant MEK 1 (T292A) (Mut). Specific immunolabeling of the ~45 kDa MEK-1 protein phosphorylated at Thr292 is shown in the second lane where MAP kinase was coexpressed.

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Rat

£454.50

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Western blot analysis of 3T3-EGF cells using Phospho-MEK-1 (T386) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K1-Thr386 antibody (344-393 aa) (STJ90585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK 1 protein phosphorylated at Thr386 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Chicken/Non-Human Primates/Mouse/Xenopus

£454.50

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Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (-) or incubated in the presence of serum (+). Immunolabeling of an additional band at ~95 kDa was also observed.

Anti-Phospho-MAP2K1/2-Ser218/222 antibody (STJA0003683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Human/Bovine/Canine/Chicken/Non-Human Primates/Rat/Xenopus/Zebrafish

£454.50

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Western blot analysis of various cells using Phospho-MEK-3 (S218) Polyclonal Antibody

Anti-Phospho-MAP2K3-Ser218 antibody (173-222 aa) (STJ90330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K3-Thr222 antibody (188-237 aa) (STJ90949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-MAP2K4-S257 Rabbit pAb (STJ116981). HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-MAP2K4-S257 antibody (STJ22241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-MAP2K4-S257 antibody [SRM13701] (STJ11108431)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-MAP2K4-S257/T261 Rabbit pAb (STJ110871) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K4-S257/T261 antibody (STJ110871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 and C6 cells, using Phospho-MAP2K4-S80 Rabbit pAb (STJ22240) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight or treated by PDGF (50 ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 150s.

Anti-Phospho-MAP2K4-S80 antibody (STJ22240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-MAP2K4-Ser257 antibody (STJ194742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-MAP2K4-Ser257/Thr261 antibody (STJ194743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-MEK-4 (S80) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAP2K4-Ser80 antibody (46-95 aa) (STJ90331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-MAP2K4-Ser80 antibody (STJ194744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-MAP2K4-T261 pAb (STJ22242) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAP2K4-T261 antibody (STJ22242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-MEK-4 (T261) Polyclonal Antibody

Anti-Phospho-MAP2K4-Thr261 antibody (227-276 aa) (STJ90332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-MAP2K4-Thr261 antibody (STJ194745)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HL60 cells, using Phospho-MEK6-S207 Rabbit pAb (STJ22247). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K6-S207 antibody (STJ22247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MAP2K6-Ser207 antibody (STJ90333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-MAP2K7-S271/T275 antibody (STJ11100969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody

Anti-Phospho-MAP2K7-Ser271 antibody (236-285 aa) (STJ90950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-MAP2K7-Ser271/Thr275 antibody (STJ194725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MAP2K7 (Phospho-Thr275) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAP2K7-Thr275 antibody (241-290 aa) (STJ90586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of NIH-3T3 cells using Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP3K1-Thr1402 antibody (1368-1417 aa) (STJ90946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-TAK1-S412 Rabbit pAb (STJ22254) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 120s.

Anti-Phospho-MAP3K7-S412 antibody (STJ22254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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NLRC5 Deficiency Reduces LPS-Induced Microglial Activation via Inhibition of NF-κB Signaling and Ameliorates Mice’s Depressive-like Behavior. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Jianqiong Zhang WB mouse 1:500 BV2 cell

Anti-Phospho-MAP3K7-Ser412 antibody (STJ196446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T using p-Tak1 (S439) antibody. Antibody was diluted at 1:500

Anti-Phospho-MAP3K7-Ser439 antibody (411-460 aa) (STJ90947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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