• Anti-MMP9 antibody [5G3] (STJ98248)
  • Flow cytometric analysis of Hela cells using MMP-9 monoclonal antibody (blue) and negative control (red).
  • Immunohistochemistry analysis of paraffin-embedded brain tissues with DAB staining using MMP-9 monoclonal antibody.
  • Immunofluorescence analysis of NIH/3T3 cells using MMP-9 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using MMP-9 monoclonal antibody against HEK293 (1) , MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.

Anti-MMP9 antibody [5G3] (STJ98248)

SKU:
STJ98248

Current Stock:
Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Matrix metalloproteinase-9 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5G3
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP9
Gene ID: 4318
Uniprot ID: MMP9_HUMAN
Specificity: MMP-9 Monoclonal Antibody detects endogenous levels of MMP-9 protein.
Immunogen: Purified recombinant fragment of human MMP-9 expressed in E. Coli.
Post Translational Modifications Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
Function Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Protein Name Matrix Metalloproteinase-9
Mmp-9
92 Kda Gelatinase
92 Kda Type Iv Collagenase
Gelatinase B
Gelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9
Database Links Reactome: R-HSA-1433557
Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-2022090
Reactome: R-HSA-3928665
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Reactome: R-HSA-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-9 antibody
Anti-Mmp-9 antibody
Anti-92 Kda Gelatinase antibody
Anti-92 Kda Type Iv Collagenase antibody
Anti-Gelatinase B antibody
Anti-Gelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 antibody
Anti-MMP9 antibody
Anti-CLG4B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance