Immune System | Research Area | St John's Laboratory

Western blot analysis of various lysates, using NDUFC2 Rabbit monoclonal antibody (STJ11104184) at1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NDUFC2 antibody (1-119) [S4184RM] (STJ11104184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NDUC2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NDUFC2 antibody (51-100) (STJ94381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-Nectin-2 antibody [502-57] (STJ16100216)

Clonality :

Monoclonal

Host :

Rat

Applications :

IHC-F/FC/ELISA/IP/WB

Reactivity :

Mouse/Human

£883.50

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Western blot analysis of mouse brain cells using Nectin 2 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-NECTIN2 antibody (151-200 aa) (STJ97298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Nectin 2/CD112 antibody (STJ27331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-NECTIN2 antibody (382-479) (STJ27331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using Nectin 2 Rabbit mAb (STJ11102509) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-NECTIN2 antibody (410-538) [S9MR] (STJ11102509)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit monoclonal antibody (STJ11102065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NEDD4 antibody (1200-1300) [S5MR] (STJ11102065)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human gastric using NEDD4 antibody (STJ24729) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NEDD4 antibody (160-460) (STJ24729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-NEDD4 antibody (300-350) (STJ506276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-NEDD4 antibody (300-350) {Biotin} (STJ506277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-NEDD4 antibody (300-350) {FITC} (STJ506278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NEDD4 antibody (80-130 aa) (STJ195353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NEDD4 antibody [PT1292R] (STJA0040043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NEDD4L antibody (28-78 aa) (STJ193468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using NEDD4L Rabbit pAb (STJ110385) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NEDD4L antibody (836-955) (STJ110385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using NEDD4L Rabbit mAb (STJ11103647) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NEDD4L antibody (876-975) [S7MR] (STJ11103647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-NEK2 antibody (STJ191974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-NEU1 antibody (139-189) (STJA0006640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using NEU1 Rabbit polyclonal antibody (STJ28221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NEU1 antibody (210-415) (STJ28221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NEU1/Sialidase-1 antibody (Ala47-Leu415) [1A147] (STJA0021972)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£229.50 - £328.50

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Anti-NEU1/Sialidase-1 antibody [1A039] (STJA0019915)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£229.50 - £328.50

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Anti-NEU1/Sialidase-1 antibody [1A040] (STJA0019916)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£229.50 - £328.50

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Anti-Neural Cell Adhesion Molecule antibody [RNL-1] (STJ16100890)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB

Reactivity :

Human

£518.50

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Western blot analysis of various lysates using Neutrophil Elastase (ELANE) Rabbit mAb (STJ11103639) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Neutrophil Elastase antibody (167-267) [S9MR] (STJ11103639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Anti-NF Kappa B P105 antibody (741-968 aa) [SRM] (STJ11107156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Western blot analysis of NF-Kappa B p65 at dilution of 1:1000

Anti-NF-Kappa B p65 antibody [SM0435] (STJA0010435)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunoprecipitation analysis of 200 Mu g extracts of Hela cells using 3 Mu g NF2 Rabbit polyclonal antibody (STJ28273). Western blot was performed from the immunoprecipitate using NF2 Rabbit polyclonal antibody (STJ28273) at a dilition of 1:1000.

Anti-NF2 antibody (19-595) (STJ28273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using Merlin antibody (STJ11104879) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NF2 antibody (347-595) (STJ11104879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ114897). Western blot was performed from the immunoprecipitate using NF2 antibody (STJ114897) at a dilution of 1:1000.

Anti-NF2 antibody (477-576) (STJ114897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using NF2 Polyclonal Antibody diluted at 1：500

Anti-NF2 antibody (485-534) (STJ94445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NF2 antibody [PT0952R] (STJA0039816)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-NF2/Merlin antibody (347-595 aa) [SRM] (STJ11107299)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NFAM1 antibody (30-110 aa) (STJ190374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NFASC antibody (STJ24750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NFASC antibody (830-1100) (STJ24750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NFASC antibody (STJA0007413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of NFAT2 in paraffin-embedded human appendix using NFAT2 Rabbit polyclonal antibody (STJ11102802) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NFAT2 antibody (115-298) (STJ11102802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates, using NFAT2 Rabbit monoclonal antibody (STJ11105126) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-NFATC1 antibody (10-305) [S5126RM] (STJ11105126)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit polyclonal antibody (STJ24751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFATC1 antibody (20-300) (STJ24751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using NFAT2 antibody (STJ11101721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFATC1 antibody (844-943) [S1MR] (STJ11101721)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of HuvEc cells using NFATc1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NFATC1 antibody (881-930 aa) (STJ96746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NFATC2 antibody (STJ116122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NFATC2 antibody (1-300) (STJ116122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of rat spleen using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFATC2 antibody (1-300) (STJ24752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-colon, antibody was diluted at 1:100

Anti-NFATC2 antibody (640-700 aa) (STJ98986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NFATC3 antibody (STJ28749) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFATC3 antibody (1-290) (STJ28749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using NFATc3 Polyclonal Antibody

Anti-NFATC3 antibody (131-180) (STJ94448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Whole cell lysates of U2OS were separated by 12% SDS-PAGE, and the membrane was blotted with Nrf2 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Lane 1: U2OSPredicted band size: 68kDaObserved band size: 65kDa

Anti-NFE2L2 antibody (300-400) [PTR2557] (STJA0006234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1：1000

Anti-NFE2L2 antibody (556-605) (STJ94554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) MCF7 Cell, 2) Mouse Brain Tissue lysate using NF κB P105/P50 mouse Monoclonal monoclonal antibody diluted at 1:1,000.

Anti-NFKB P105/P50 antibody [4D11] (STJ196682)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse

£174.00 - £252.00

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