• Western blot analysis of U-937, using IL13 rabbit polyclonal antibody (STJ24163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
  • Western blot analysis of K-562, using IL13 rabbit polyclonal antibody (STJ24163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using IL13 antibody (STJ24163) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using IL13 antibody (STJ24163) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using IL13 antibody (STJ24163) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IL13 antibody (25-146) (STJ24163)

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STJ24163

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL13 (25-146) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL13
Gene ID: 3596
Uniprot ID: IL13_HUMAN
Immunogen Region: 25-146
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-146 of human IL13 (NP_002179.2).
Immunogen Sequence: LTCLGGFASPGPVPPSTALR ELIEELVNITQNQKAPLCNG SMVWSINLTAGMYCAALESL INVSGCSAIEKTQRMLSGFC PHKVSAGQFSSLHVRDTKIE VAQFVKDLLLHLKKLFREGQ FN
Function Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B. Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6. Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13.
Protein Name Interleukin-13
Il-13
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-9012546
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-13 antibody
Anti-Il-13 antibody
Anti-IL13 antibody
Anti-NC30 antibody

Information sourced from Uniprot.org

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