Haemostasis | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ARRB1-Ser412 antibody (369-418 aa) (STJ90802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using p130 Cas (Phospho-Tyr165) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BCAR1-Tyr165 antibody (131-180 aa) (STJ90857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30', using p130 Cas (Phospho-Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BCAR1-Tyr249 antibody (215-264 aa) (STJ90858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-BCAR1-Tyr410 antibody (376-425 aa) (STJ90765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from PC-12 cells, using Phospho-BCAR1-Y165 Rabbit pAb (STJ117911) at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-BCAR1-Y165 antibody (STJ117911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (STJ1111150). HeLa cells were treated by Pervanadate (1 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-BCAR1-Y410 antibody (STJ11101096)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit pAb (STJ117887) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-BCAR1-Y410 antibody (STJ117887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Phospho-beta Arrestin 1 Ser412 antibody western blot A549 MCF-7 U251 HL-60 STJA0035053

Anti-Phospho-beta Arrestin 1-Ser412 antibody [R05-6F7] (STJA0035053)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Beta-Tubulin-Ser-172 antibody (STJA0041423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Phospho-c-Myb Ser11 antibody western blot Jurkat STJA0034999

Anti-Phospho-c Myb-Ser11 antibody [R04-2D3] (STJA0034999)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ChIP

Reactivity :

Human

£133.50 - £281.50

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Western blot K-562 cells c-Abl phosphorylation detection using antibody STJA0041063

Anti-Phospho-c-Abl-Tyr-245 antibody (STJA0041063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot K-562 cells c-Abl phosphorylation detection using antibody STJA0041064

Anti-Phospho-c-Abl-Tyr-412 antibody (STJA0041064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot C-Raf detection in Jurkat cells with phosphatase treatment comparison STJA0041368

Anti-Phospho-C-Raf-Ser-471 [S578/S433] antibody (STJA0041368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot c-Src phosphorylation in mouse SYF cells with pervanadate treatment STJA0041394

Anti-Phospho-c-Src-Tyr-215 antibody (STJA0041394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot analysis c-Src phosphorylation in SYF cells using antibody STJA0041379

Anti-Phospho-c-Src-Tyr-530 antibody (C-Term) [M261] (STJA0041379)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-CAV1-Tyr-14 antibody [M283] (STJA0005314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis of various cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CAV1-Tyr14 antibody (5-54 aa) (STJ90210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using Phospho-Caveolin-1-Y14 Rabbit pAb (STJ116375) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Hydrogen Peroxide (2 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-CAV1-Y14 antibody (STJ116375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis caveolin-1 antibody in human A431 cells with pervanadate stimulation STJA0041116

Anti-Phospho-Caveolin-1-Tyr-14 antibody [M283] (STJA0041116)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£579.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HP1 alpha (Phospho-Ser92) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CBX5-Ser92 antibody (58-107 aa) (STJ91233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Phospho-CD18 antibody western blot K562 rat brain C6 3T3 STJA0034131

Anti-Phospho-CD18-Ser745 antibody [R01-4B6] (STJA0034131)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of 3T3 cells using Phospho-CD44 (S706) Polyclonal Antibody

Anti-Phospho-CD44-Ser706 antibody (681-730 aa) (STJ90509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells, using Phospho-CDK2-T160 Rabbit pAb (STJ22062) at 1:400 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK2-T160 antibody (STJ22062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit monoclonal antibody (STJ11105902) at 1:1000 dilution. C6 cells were treated by Nocodazole (10ng/ml). Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CDK2-T160 antibody [S5902RM] (STJ11105902)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£143.50 - £618.50

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Anti-Phospho-CDK2-T160-T160 antibody [SRM15122] (STJ11109852)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Phospho-CDK2 Thr14 antibody western blot HeLa A549 HL-60 U2OS STJA0035056

Anti-Phospho-CDK2-Thr14 antibody [R06-4C1] (STJA0035056)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CDK2-Thr160 antibody (STJ90752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-CDK2 Tyr15 antibody western blot C6 UV lysates STJA0033906

Anti-Phospho-CDK2-Tyr15 antibody [R05-2C4] (STJA0033906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

£133.50 - £281.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK2-Y15 Rabbit mAb (STJ11102594) at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours or treated by Nocodazole (100 ng/mL) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CDK2-Y15 antibody [S4MR] (STJ11102594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western Blot analysis of mouse lung tissue, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CDK5-Ser159 antibody (STJA0009913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody

Anti-Phospho-CDK5-Tyr15 antibody (1-50 aa) (STJ90540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CFL1-S3 Rabbit pAb (STJ26176). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CFL1-S3 antibody (STJ22068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-CFL1-Ser-3 antibody (STJA0005346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western blot analysis of NIH-3T3 cells using Phospho-Cofilin (S3) Polyclonal Antibody

Anti-Phospho-CFL1-Ser3 antibody (1-50 aa) (STJ90230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (STJ11105777) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-CFL1-Y139 antibody (STJ11105777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot cofilin phosphorylation in Jurkat cells with phosphatase treatment STJA0041140

Anti-Phospho-Cofilin 1-Ser-3 antibody (STJA0041140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Anti-Phospho-Cofilin antibody (1-50) (STJ506121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Phospho-Cofilin antibody (1-50) {Biotin} (STJ506122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-Cofilin antibody (1-50) {FITC} (STJ506123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-Crk II-Tyr-221 antibody (STJA0041170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Western blot Crk Tyr-251 phospho-specific antibody detection in K562 cells STJA0041160

Anti-Phospho-Crk II-Tyr-251 antibody (STJA0041160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Anti-Phospho-CRK-Tyr-221 antibody (STJA0005354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-CRK-Tyr-251 antibody (STJA0005355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Anti-Phospho-CRK-Tyr221 antibody (187-236 aa) (STJ90687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Phospho-CrkII antibody western blot K562 lysates STJA0036028

Anti-Phospho-CRK-Tyr221 antibody [R04-6L8] (STJA0036028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from K-562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (STJ117920) at 1:1000 dilution. K-562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody (STJ117920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (STJ11102643) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody [S3MR] (STJ11102643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of VEC cells using Phospho-Csk (S364) polyclonal antibody STJ90456

Anti-Phospho-CSK-Ser364 antibody (330-379 aa) (STJ90456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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