Anti-Phospho-Crk II-Tyr-251 antibody (STJA0041160)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Crk II-Tyr-251 for use in ELISA, WB and IHC in Chicken, Human, Mouse, Rat and Xenopus samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/IHC |
| Host | Rabbit |
| Reactivity | Chicken/Human/Mouse/Rat/Xenopus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | CRK |
| Gene ID | 1398 |
| Uniprot ID | CRK_HUMAN |
| Immunogen | Phospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in |
| Immunogen Sequence | Human |
| Specificity | The antibody detects a 38 and 40 kDa* proteins corresponding to the molecular mass of Crk II and CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after ak |
Additional Info
| Post Translational Modifications | Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form. |
| Function | Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Protein Name | Adapter Molecule CrkProto-Oncogene C-CrkP38 |
| Database Links | Reactome: R-HSA-170984Reactome: R-HSA-186763Reactome: R-HSA-2029482Reactome: R-HSA-372708Reactome: R-HSA-4420097Reactome: R-HSA-8849471Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-912631Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCell MembraneTranslocated To The Plasma Membrane Upon Cell Adhesion |
| Alternative Antibody Names | Anti-Adapter Molecule Crk antibodyAnti-Proto-Oncogene C-Crk antibodyAnti-P38 antibodyAnti-CRK antibody |
Information sourced from Uniprot.org