Gene Expression And Transcription | Research Area | St John's Laboratory

Anti-SMAD4 antibody (136-156) {FITC} (STJ502007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Immunofluorescence analysis of Hela cell. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD4 antibody (21-70 aa) (STJ95702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Smad4 antibody immunocytochemistry HeLa cells STJA0012592

Anti-SMAD4 antibody (316-552 aa) [R05-1I7] (STJA0012592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from HCT116 cells, using Smad4 Rabbit pAb (STJ27624) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SMAD4 antibody (320-552) (STJ27624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Smad4 rabbit monoclonal antibody (STJ11101653) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB) , as shown in figure.

Anti-SMAD4 antibody (320-552) [S3MR] (STJ11101653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunohistochemistry of mouse liver with Anti-SMAD4 primary antibody (STJ503060) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (400-450) (STJ503060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£400.50

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Immunohistochemistry with Anti-SMAD4 Antibody (STJ503062) at 1:50 dilution in buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 20X magnification. FFPE section.

Anti-SMAD4 antibody (400-450) (STJ503062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey/Mouse/Rat

£400.50

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Immunohistochemistry of mouse small intestine with Anti-SMAD4 primary antibody (STJ503061) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (502-552) (STJ503061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-SMAD4 antibody (502-552) {Biotin} (STJ503067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (502-552) {FITC} (STJ503068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis using Smad4 monoclonal antibody against A431 (1) , SK-N-SH (2) , K562 (3) , HepG2 (4) and HUVE12 (5) cell lysate.

Anti-SMAD4 antibody [4G1C6] (STJ98385)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Smad4 antibody immunohistochemistry paraffin-embedded tonsil tissue STJA0037716

Anti-Smad4 antibody [BP6219] (STJA0037716)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£308.50

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Anti-Smad4 antibody [PT0550R] (STJA0039429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-DPC4 (SMAD4) antibody IHC human pancreatic ductal carcinoma STJ180565

Anti-SMAD4 antibody [ZR392] (STJ180565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£148.50 - £589.50

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Western blot analysis of extracts of various cell lines, using Smad6 antibody (STJ25610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SMAD6 antibody (257-496) (STJ25610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of Smad6 antibody in paraffin-embedded human lung carcinoma tissue.

Anti-SMAD6 antibody (323-372 aa) (STJ95703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis using Smad6 monoclonal antibody against A431 (1) , A431 (2) , HeLa (3) and Jurkat (4) cell lysate.

Anti-SMAD6 antibody [5H3] (STJ98387)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-SMAD7 antibody (N-Term) (STJA0005586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£319.50

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Western blot WB analysis SMAD7 in HepG2 cells using antibody STJA0041408

Anti-SMAD7 antibody (N-Term) (STJA0041408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£474.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SMAD7 antibody (STJ192434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Goat/Sheep

£49.00 - £261.00

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Anti-Small ubiquitin-related modifier 1 antibody (aa: 6-21) (STJA0040870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£540.50

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Anti-Small ubiquitin-related modifier 1 antibody (aa: 6-21) (STJA0041027)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£540.50

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Western blot analysis of extracts from A549 cells, using Brm Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SMARCA2 antibody (1328-1377 aa) (STJ96404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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SMARCA2 antibody immunocytochemistry 293 cells STJA0012593

Anti-SMARCA2 antibody (62-77 aa) [R02-3E2] (STJA0012593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-SMARCA2 IP antibody [SAA0257] (STJA0018772)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£195.50 - £1,319.50

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Anti-SMARCA2/BRM antibody [SRM14623] (STJ11109353)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ChIP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysate from K562 cells, using Brg-1 antibody.

Anti-SMARCA4 antibody (1565-1614 aa) (STJ91889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using BRG1/SMARCA4 antibody (STJ11101695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SMARCA4 antibody (200-300) [S5MR] (STJ11101695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells using BRG1/BRG1/SMARCA4 antibody (STJ113555).

Anti-SMARCA4 antibody (30-130) (STJ113555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g BRG1/SMARCA4 antibody (STJ25612). Western blot was performed from the immunoprecipitate using BRG1/SMARCA4 antibody (STJ25612) at a dilution of 1:1000.

Anti-SMARCA4 antibody (680-770) (STJ25612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot detection of BRG1 in Hela NE, F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.

Anti-SMARCA4 antibody [6D7-F7-B6] (STJ99234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of extracts of various cell lines, using SMARCA5/SNF2H antibody (STJ25614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2min.

Anti-SMARCA5 antibody (100-400) (STJ25614)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SMARCA5 antibody (851-901 aa) (STJ194317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Confocal imaging of C2C12 cells using SMARCA5/SNF2H Rabbit monoclonal antibody (STJ11101895, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SMARCA5 antibody (953-1052) [S5MR] (STJ11101895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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SMARCA5 antibody immunocytochemistry Jurkat cells STJA0034871

Anti-SMARCA5 antibody [R05-9D5] (STJA0034871)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-INI-1 antibody IHC Human epithelioid sarcoma STJ180136

Anti-SMARCB1 antibody (257-359aa) [25] (STJ180136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£166.50 - £683.50

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LN18 whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-INI-1 (ABT-INI1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LN18Predicted band size: 44kDaObserved band size: 44kDa

Anti-SMARCB1 antibody (300-385 aa) [ABT-INI1] (STJ196996)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£0.00

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LN18 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-INI-1 (ABT188) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LN18Predicted band size: 44kDaObserved band size: 44kDa

Anti-SMARCB1 antibody (300-385 aa) [ABT188] (STJ190074)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of HEPG2 cells using Antibody diluted at 800. Secondary antibody was diluted at 1:20000

Anti-SMARCB1 antibody (331-380 aa) (STJ98643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using SMARCB1/SNF5 Rabbit polyclonal antibody (STJ11104571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMARCB1 antibody (50-150) (STJ11104571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g SMARCB1/SNF5 antibody (STJ28334). Western blot was performed from the immunoprecipitate using SMARCB1/SNF5 antibody (STJ28334) at a dilution of 1:1000.

Anti-SMARCB1 antibody (50-150) (STJ28334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using SMARCB1/SNF5 rabbit monoclonal antibody (STJ11101841) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:2000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMARCB1 antibody (50-150) [S1MR] (STJ11101841)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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SMARCB1 antibody immunohistochemistry paraffin-embedded Astrocytoma STJA0033539

Anti-SMARCB1 antibody [5F1-6F6-3A1] (STJA0033539)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£214.50 - £308.50

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SMARCB1 antibody immunohistochemistry colon cancer paraffin-embedded STJA0037744

Anti-SMARCB1 antibody [R02-3N3] (STJA0037744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£281.50

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