Anti-SMARCA2/BRM antibody [SRM14623] (STJ11109353)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 201-300 of human SMARCA2 (NP_003061.3).
General Information
| Short Description | Rabbit monoclonal SMARCA2/BRM antibody for use in WB, IHC-P, IF, ICC, IP, ChIP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ChIP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14623 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:500-1:2000IHC-P, 1:200-1:800IF/ICC, 1:50-1:200IP, 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsChIP, 5 Mu g antibody for 10 Mu g-20 Mu g of ChromatinELISA, Recommended starting concentration is |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMARCA2 |
| Gene ID | 6595 |
| Uniprot ID | SMCA2_HUMAN |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SMARCA2 (NP_003061.3). |
Additional Info
| Post Translational Modifications | During apoptosis, cleaved by cathepsin CTSG to produce a 160 kDa cleavage product which localizes to the cytosol. Ubiquitinated. |
| Function | ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. |
| Protein Name | Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2Samrca2Brg1-Associated Factor 190bBaf190bProbable Global Transcription Activator Snf2l2Protein Brahma HomologHbrmSnf2-Alpha |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8939243Reactome: R-HSA-9824585Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2 antibodyAnti-Samrca2 antibodyAnti-Brg1-Associated Factor 190b antibodyAnti-Baf190b antibodyAnti-Probable Global Transcription Activator Snf2l2 antibodyAnti-Protein Brahma Homolog antibodyAnti-Hbrm antibodyAnti-Snf2-Alpha antibodyAnti-SMARCA2 antibodyAnti-BAF190B antibodyAnti-BRM antibodyAnti-SNF2A antibodyAnti-SNF2L2 antibody |
Information sourced from Uniprot.org