Anti-SMAD6 antibody (257-496) (STJ25610)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Smad6 (257-496) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMAD6 |
| Gene ID | 4091 |
| Uniprot ID | SMAD6_HUMAN |
| Immunogen Region | 257-496 |
| Immunogen Sequence | PTVCCNPYHFSRLCGPESPP PPYSRLSPRDEYKPLDLSDS TLSYTETEATNSLITAPGEF SDASMSPDATKPSHWCSVAY WEHRTRVGRLYAVYDQAVSI FYDLPQGSGFCLGQLNLEQR SESVRRTRSKIGFGILLSKE PDGVWAYNRGEHPIFVNSPT LDAPGGRALVVRKVPPGYSI KVFDFERSGLQHAPEPDAAD GPYDPNSVRISFAKGWGPCY SRQFITSCPCWLEILLNNP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 257-496 of human Smad6 (NP_005576.3). |
Additional Info
| Tissue Specificity | Isoform B: Expressed in the brain, heart, ovary, peripheral blood leukocytes, small intestine, spleen, thymus, bone marrow, fetal liver and lymph nodes. |
| Post Translational Modifications | Phosphorylated by BMP type 1 receptor kinase and by PRKX. Monoubiquitinated at Lys-173 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation. Ubiquitinated by WWP1. Ubiquitinated by ARK2C, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling. Arginine methylation by PRMT1, which is recruited by BMPR2, initiates BMP-Induced signaling and induces dissociation from the BMPR1B receptor at the cell surface leading to derepress downstream Smad1/Smad5 signaling. |
| Function | Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta. Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of pro-inflammatory genes. Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions. |
| Protein Name | Mothers Against Decapentaplegic Homolog 6Mad Homolog 6Mothers Against Dpp Homolog 6Smad Family Member 6Smad 6Smad6Hsmad6 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-8941326 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 6 antibodyAnti-Mad Homolog 6 antibodyAnti-Mothers Against Dpp Homolog 6 antibodyAnti-Smad Family Member 6 antibodyAnti-Smad 6 antibodyAnti-Smad6 antibodyAnti-Hsmad6 antibodyAnti-SMAD6 antibodyAnti-MADH6 antibody |
Information sourced from Uniprot.org