Anti-SMARCA5 antibody [R05-9D5] (STJA0034871)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human SNF2H
General Information
| Short Description | Rabbit monoclonal anti-SMARCA5 for use in WB, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-9D5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SMARCA5 |
| Gene ID | 8467 |
| Uniprot ID | SMCA5_HUMAN |
| Immunogen | A synthetic peptide of human SNF2H |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Function | ATPase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair.this may require intact histone H4 tails. Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template. Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes. The BAZ1A/ACF1-, BAZ1B/WSTF-, BAZ2A/TIP5- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner. The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template. Binds to core histones together with RSF1, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex. Involved in DNA replication and together with BAZ1A/ACF1 is required for replication of pericentric heterochromatin in S-phase. Probably plays a role in repression of RNA polymerase I dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH-5 ISWI chromatin-remodeling complex (also called the WICH complex), a chromatin-remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription. Within the B-WICH complex has a role in RNA polymerase III transcription. Mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of NoRC-5 ISWI chromatin-remodeling complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. |
| Protein Name | Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 5Smarca5Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin A5Sucrose Nonfermenting Protein 2 HomologHsnf2h |
| Database Links | Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-5693565Reactome: R-HSA-606279 |
| Cellular Localisation | NucleusChromosomeLocalizes To Mitotic ChromosomesCo-Localizes With Rsf1 In The NucleusCo-Localizes With Pcna At Replication Foci During S PhaseCo-Localizes With Baz1b/Wstf At Replication Foci During Late-S PhaseRecruited To Dna Damage Sites Following Interaction With Sirt6 |
| Alternative Antibody Names | Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 5 antibodyAnti-Smarca5 antibodyAnti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin A5 antibodyAnti-Sucrose Nonfermenting Protein 2 Homolog antibodyAnti-Hsnf2h antibodyAnti-SMARCA5 antibodyAnti-SNF2H antibodyAnti-WCRF135 antibody |
Information sourced from Uniprot.org