DNA Repair | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using XPC antibody (STJ110652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-XPC antibody (700-940) (STJ110652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of XPD/ERCC2 in paraffin-embedded human colon carcinoma tissue using XPD/ERCC2 Rabbit monoclonal antibody (STJ11102973) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-XPD/ERCC2 antibody (600-700) [S3MR] (STJ11102973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using XRCC1 Rabbit monoclonal antibody (STJ11102000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-XRCC1 antibody (1-100) [S0MR] (STJ11102000)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of HeLa cells using XRCC1 antibody (STJ26128) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-XRCC1 antibody (480-633) (STJ26128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using XRCC1 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-XRCC1 antibody (517-566 aa) (STJ96282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of XRCC1 in Raji, K562, Hela, Jurkat and THP-1 cell lysates using XRCC1 mouse mAb (1:1000 diluted).Predicted band size:82KDa.Observed band size:82KDa.

Anti-XRCC1 antibody [3F6-G7-F7] (STJ99239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Anti-XRCC2 antibody (1-280) (STJ115598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-XRCC2 antibody (1-83 aa) [SRM] (STJ11107260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded rat lung using XRCC2 Rabbit polyclonal antibody (STJ11103888) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-XRCC2 antibody (1-83) (STJ11103888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using XRCC2 Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-XRCC2 antibody (211-260 aa) (STJ96283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using XRCC3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-XRCC3 antibody (41-90 aa) (STJ96284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (STJ26131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-XRCC3 antibody (62-346) (STJ26131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and XRCC4 knockout (KO) HeLa cells, using [KO Validated] XRCC4 Rabbit polyclonal antibody (STJ11100019) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-XRCC4 antibody (1-336) (STJ11100019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of 293T cells using XRCC4 Rabbit polyclonal antibody (STJ26132) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-XRCC4 antibody (1-336) (STJ26132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of various cells using XRCC4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-XRCC4 antibody (261-310 aa) (STJ96286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Bak Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-XRCC4 antibody [5C10] (STJ96988)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various cells using Ku-86 Polyclonal Antibody

Anti-XRCC5 antibody (441-490 aa) (STJ93869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Ku80 antibody (STJ28425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-XRCC5 antibody (543-732) (STJ28425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Ku80 antibody (STJ11101409). Western blot was performed from the immunoprecipitate using Ku80 antibody (STJ11101409) at a dilution of 1:1000.

Anti-XRCC5 antibody (633-732) [S9MR] (STJ11101409)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from 1) K562, 2) Jurkat, 3) COS7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-XRCC5 antibody (683-732 aa) (STJ93866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Anti-XRCC5 antibody (Internal Region) (STJA0005773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Hamster/Human/Mouse/Rat

£455.50

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Anti-XRCC5 antibody (Internal Region) (STJA0005774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Hamster/Human/Mouse/Rat

£455.50

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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-XRCC5 antibody (STJ193184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using Ku-80 monoclonal antibody against HeLa (1) , MCF-7 (2) , A549 (3) and NIH/3T3 (4) cell lysate.

Anti-XRCC5 antibody [5C5] (STJ98211)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot detection of Ku80 in COS7, Jurkat, Hela, MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.

Anti-XRCC5 antibody [8H1-C3-G10] (STJ99226)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Anti-XRCC5 Ku-86 kd subunit antibody [J15] (STJ16102618)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Monkey

£287.50 - £1,324.50

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Confocal immunofluorescence analysis of U2OS cells using Ku70 Polyclonal Antibody (STJ26134) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-XRCC6 antibody (408-609) (STJ26134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of U-2 OS cells using Ku70 Rabbit monoclonal antibody (STJ11101297, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-XRCC6 antibody (500-609) [S7MR] (STJ11101297)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunoprecipitation analysis of 100 Mu g extracts of SW480 cells using 3 Mu g Ku70 antibody (STJ29469). Western blot was performed from the immunoprecipitate using Ku70 antibody (STJ29469) at a dilution of 1:1000.

Anti-XRCC6 antibody (501-600) (STJ29469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using [KD Validated] Ku70 Rabbit polyclonal antibody (STJ11105550) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-XRCC6 antibody (505-609) (STJ11105550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Ku-70 Polyclonal Antibody

Anti-XRCC6 antibody (560-609 aa) (STJ93863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Ku70 in Hela, A549, COS7 and K562 cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.

Anti-XRCC6 antibody [7B1-E12-G4] (STJ99222)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (STJ11101704) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-YY1 antibody (200-300) [S4MR] (STJ11101704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-YY1 antibody (230-310 aa) (STJ192391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (STJ114794) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-YY1 antibody (80-160) (STJ114794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ZNF830 antibody (STJA0009355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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beta Actin Blocking Peptide peptide (STJ505544)

Applications :

Immunodepletion/Immunocompetition

£281.50

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beta Actin Blocking Peptide peptide (STJ505545)

Applications :

Immunodepletion/Immunocompetition

£281.50

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beta Actin Positive Control peptide (STJ505546)

Applications :

WB

£331.50

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CCDC98 Blocking Peptide for STJ500022 peptide (STJ503617)

Applications :

Immunodepletion/Immunocompetition

£281.50

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CCDC98 Positive Control for STJ500026 peptide (STJ503618)

Applications :

WB

£331.50

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DNA PKcs Blocking Peptide peptide (STJ503959)

Applications :

Immunodepletion/Immunocompetition

£281.50

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DNA PKcs Positive Control peptide (STJ503979)

Applications :

WB

£331.50

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ETS1 associated protein II Blocking Peptide peptide (STJ504983)

Applications :

Immunodepletion/Immunocompetition

£281.50

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ETS1 associated protein II Positive Control peptide (STJ505190)

Applications :

WB

£331.50

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Human ACTb (Actin Beta) Sandwich ELISA Kit Kit (STJE0006866)

Applications :

ELISA

Reactivity :

Human

£446.50 - £611.50

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Human ACTb (Actin Beta) Sandwich ELISA Kit Ready-To-Use Kit (STJE0013242)

Applications :

ELISA

Reactivity :

Human

£466.50 - £637.50

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Human APEX1 (APEX Nuclease 1) Sandwich ELISA Kit Kit (STJE0007208)

Applications :

ELISA

Reactivity :

Human

£465.50 - £636.50

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