• Western blot analysis of various lysates, using Rad51 antibody (STJ28190) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of C6, using Rad51 antibody (STJ28190) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using Rad51 rabbit polyclonal antibody (STJ28190) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using Rad51 rabbit polyclonal antibody (STJ28190) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-RAD51 antibody (1-339) (STJ28190)

SKU:
STJ28190

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Rad51 (1-339) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD51
Gene ID: 5888
Uniprot ID: RAD51_HUMAN
Immunogen Region: 1-339
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human Rad51 (NP_002866.2).
Immunogen Sequence: MAMQMQLEANADTSVEEESF GPQPISRLEQCGINANDVKK LEEAGFHTVEAVAYAPKKEL INIKGISEAKADKILAEAAK LVPMGFTTATEFHQRRSEII QITTGSKELDKLLQGGIETG SITEMFGEFRTGKTQICHTL AVTCQLPIDRGGGEGKAMYI DTEGTFRPERLLAVAERYGL SGSDVLDNVAYARAFNTDHQ TQLLYQASAMMVESRYALLI VDSATALYRTDYSGRGELS
Tissue Specificity Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Post Translational Modifications Ubiquitinated by the SCF(FBH1) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA. Ubiquitinated by RFWD3 in response to DNA damage: ubiquitination leads to degradation by the proteasome, promoting homologous recombination. Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
Function Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair.
Protein Name Dna Repair Protein Rad51 Homolog 1
Hsrad51
Hrad51
Rad51 Homolog A
Database Links Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693616
Reactome: R-HSA-8953750
Reactome: R-HSA-912446
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Mitochondrion Matrix
Chromosome
Cytoskeleton
Microtubule Organizing Center
Centrosome
Colocalizes With Rad51ap1 And Rpa2 To Multiple Nuclear Foci Upon Induction Of Dna Damage
Dna Damage Induces An Increase In Nuclear Levels
Together With Fignl1
Redistributed In Discrete Nuclear Dna Damage-Induced Foci After Ionizing Radiation (Ir) Or Camptothecin (Cpt) Treatment
Accumulated At Sites Of Dna Damage In A Spidr-Dependent Manner
Recruited At Sites Of Dna Damage In A Mcm9-Mcm8-Dependent Manner
Colocalizes With Ercc5/Xpg To Nuclear Foci In S Phase
Recruited To Stalled Replication Forks During Replication Stress By The Tonsl-Mms22l Complex
As Well As Atad5 And Wdr48 In An Atr-Dependent Manner
Alternative Antibody Names Anti-Dna Repair Protein Rad51 Homolog 1 antibody
Anti-Hsrad51 antibody
Anti-Hrad51 antibody
Anti-Rad51 Homolog A antibody
Anti-RAD51 antibody
Anti-RAD51A antibody
Anti-RECA antibody

Information sourced from Uniprot.org

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