• Western blot analysis of extracts of various cell lines, using Rad50 Rabbit monoclonal antibody (STJ11101961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RAD50 antibody (1200-1300) [S1MR] (STJ11101961)

SKU:
STJ11101961

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Rad50 (1200-1300) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD50
Gene ID: 10111
Uniprot ID: RAD50_HUMAN
Immunogen Region: 1200-1300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human Rad50 (Q92878).
Immunogen Sequence: RCSAGQKVLASLIIRLALAE TFCLNCGIIALDEPTTNLDR ENIESLAHALVEIIKSRSQQ RNFQLLVITHDEDFVELLGR SEYVEKFYRIKKNIDQCSEI V
Tissue Specificity Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Protein Name Dna Repair Protein Rad50
Hrad50
Database Links Reactome: R-HSA-2559586
Reactome: R-HSA-5685938
Reactome: R-HSA-5685939
Reactome: R-HSA-5685942
Reactome: R-HSA-5693548
Reactome: R-HSA-5693554
Reactome: R-HSA-5693565
Reactome: R-HSA-5693568
Reactome: R-HSA-5693571
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Chromosome
Telomere
Localizes To Discrete Nuclear Foci After Treatment With Genotoxic Agents
Alternative Antibody Names Anti-Dna Repair Protein Rad50 antibody
Anti-Hrad50 antibody
Anti-RAD50 antibody

Information sourced from Uniprot.org

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