• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CTIP Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-RBBP8 antibody (293-342 aa) (STJ92517)

SKU:
STJ92517

Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA endonuclease RBBP8 (293-342 aa) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBBP8
Gene ID: 5932
Uniprot ID: CTIP_HUMAN
Immunogen Region: 293-342 aa
Specificity: CtIP Polyclonal Antibody detects endogenous levels of CtIP protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CTIP at the amino acid range 293-342
Post Translational Modifications Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1. Phosphorylation is not required for tetramerization. Binds to DNA more strongly when dephosphorylated. Ubiquitinated. Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteasomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control. Ubiquitinated by RNF138 at its N-terminus. Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex.this modification leads to proteasomal degradation. Ubiquitinated by the E3 FZR1/APC/C complex.this modification leads to proteasomal degradation.
Function Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations. Binds preferentially to DNA Y-junctions and to DNA substrates with blocked ends and promotes intermolecular DNA bridging.
Protein Name Dna Endonuclease Rbbp8
Ctbp-Interacting Protein
Ctip
Retinoblastoma-Binding Protein 8
Rbbp-8
Retinoblastoma-Interacting Protein And Myosin-Like
Rim
Sporulation In The Absence Of Spo11 Protein 2 Homolog
Sae2
Database Links Reactome: R-HSA-5685938
Reactome: R-HSA-5685939
Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-8953750
Reactome: R-HSA-912446
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Chromosome
Associates With Sites Of Dna Damage In S/G2 Phase
Ubiquitinated Rbbp8 Binds To Chromatin Following Dna Damage
Alternative Antibody Names Anti-Dna Endonuclease Rbbp8 antibody
Anti-Ctbp-Interacting Protein antibody
Anti-Ctip antibody
Anti-Retinoblastoma-Binding Protein 8 antibody
Anti-Rbbp-8 antibody
Anti-Retinoblastoma-Interacting Protein And Myosin-Like antibody
Anti-Rim antibody
Anti-Sporulation In The Absence Of Spo11 Protein 2 Homolog antibody
Anti-Sae2 antibody
Anti-RBBP8 antibody
Anti-CTIP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance