Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of various cells using Phospho-MEF-2 (S408) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MEF2A-Ser408 antibody (374-423 aa) (STJ91020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEF2A (Phospho-Thr312) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2A-Thr312 antibody (279-328 aa) (STJ90323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-MEF-2 (T319) Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MEF2A-Thr319 antibody (296-345 aa) (STJ90324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody (STJ22289) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MEF2C-S396 antibody (STJ22289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-MEF2C-Ser222 antibody (STJA0003682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Chicken/Guinea Pig/Non-Human Primates/Mouse/Rat/Sheep

$613.58

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Immunohistochemistry analysis of paraffin-embedded human heart, using MEF2C (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2C-Ser387 antibody (353-402 aa) (STJ91116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using MEF2C (Phospho-Ser396) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MEF2C-Ser396 antibody (362-411 aa) (STJ90982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of K562 AD293 using Phospho-MEF-2D (S444) antibody. Antibody was diluted at 1:500

Anti-Phospho-MEF2D-Ser444 antibody (410-459 aa) (STJ90325)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of HeLa cells, using Phospho-MEK1-T292 antibody (STJ11103753) at 1:1000 dilution. HeLa cells were treated by Nocodazole (100 ng/ml) at 37 °C for 16 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-MEK1-T292 antibody (STJ11103753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MET-Ser985 antibody (STJA0010082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-Met (Phospho-Tyr1003) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MET-Tyr1003 antibody (976-1025 aa) (STJ91076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Met (Y1234) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MET-Tyr1234 antibody (1201-1250 aa) (STJ90334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MET-Tyr1234/1235 antibody (STJ196357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MET-Tyr1235 antibody (STJA0009841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of 1 HeLa cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MET-Tyr1313 antibody (STJA0010050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Met (Y1349) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MET-Tyr1349 antibody (1316-1365 aa) (STJ90335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 3T3 cells using Phospho-Met (Y1356) Polyclonal Antibody

Anti-Phospho-MET-Tyr1356 antibody (1331-1380 aa) (STJ90469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of 1 HeLa cell, HepG2 cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MET-Tyr1365 antibody (STJA0010033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-MET-Y1003 antibody (STJ22290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-MET-Y1234 antibody (STJ22292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1234/1235 antibody (STJ11100968) at 1:1000 dilution. HeLa cells were treated by HGF (40 ng/ml) at 37 °C for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-MET-Y1234/1235 antibody (STJ11100968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 antibody (STJ11105828) at1:2000 dilution. C2C12 and C6 cells were treated by HGF (40 ng/ml) at 37 °C for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-MET-Y1234/Y1235 antibody [S5828RM] (STJ11105828)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Phospho-MET-Y1235 antibody (STJ22293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1349 antibody (STJ22291) at 1:1000 dilution. HeLa cells were treated by HGF (40ng/mL) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-MET-Y1349 antibody (STJ22291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human heart, using MYB (Phospho-Ser12) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYB-Ser12 antibody (1-50 aa) (STJ90821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myb (Phospho-Ser532) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYB-Ser532 antibody (496-545 aa) (STJ91262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts from A431 cells using Phospho-c-Myc-S373 antibody (STJ22301). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MYC-S373 antibody (STJ22301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-c-Myc-S62 antibody (STJ22299) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-MYC-S62 antibody (STJ22299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Phospho-c-Myc-S62 rabbit monoclonal antibody (STJ11102580) at 1:1000 dilution. 293T cells were treated by Forskolin (10 uM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-MYC-S62 antibody [S0MR] (STJ11102580)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser373) Antibody.

Anti-Phospho-MYC-Ser373 antibody (340-389 aa) (STJ90227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-c-Myc (S62) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MYC-Ser62 antibody (31-80 aa) (STJ91021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from HT29 cells untreated or treated with UV, using Phospho-c-Myc-T358 antibody (STJ22303). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MYC-T358 antibody (STJ22303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of extracts of 293T cells, using Phospho-c-Myc-T58 antibody (STJ22298) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-MYC-T58 antibody (STJ22298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of HeLa cells, using Phospho-c-Myc-T58 rabbit monoclonal antibody (STJ11102581) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MYC-T58 antibody [S1MR] (STJ11102581)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of extracts of C6 cells, using Phospho-c-Myc-T58/S62 rabbit monoclonal antibody (STJ11102579) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MYC-T58/S62 antibody [S9MR] (STJ11102579)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$197.78 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Thr358) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYC-Thr358 antibody (325-374 aa) (STJ90228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1：500

Anti-Phospho-MYC-Thr58 antibody (25-74 aa) (STJ90229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-MYF5-Ser49 antibody (21-70 aa) (STJ90555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of 293T cells, using Phospho-MYH9-S1943 antibody (STJ117900) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-Phospho-MYH9-S1943 antibody (STJ117900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-MYH9-S1943 antibody (STJA0005473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Anti-Phospho-MYH9-Ser-1803 antibody (STJA0005471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Anti-Phospho-MYH9-Ser-1916 antibody (STJA0005472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MYH9-Ser1943 antibody (STJ196359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-MYH9-Ser1943 antibody (STJ196360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from 293 cells untreated or treated with starvation using Phospho-MYL12A-S18 antibody (STJ22304). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MYL12A-S18 antibody (STJ22304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$174.83 - $867.38

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Anti-Phospho-MYL12A-Ser-1 antibody (STJA0005479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Bovine

$496.13

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Anti-Phospho-MYL12A-Ser-19 antibody (STJA0005480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Western blot analysis of extracts of C6 cells, using Phospho-MYL9-S19 antibody (STJ11101010) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-MYL9-S19 antibody (STJ11101010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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