Anti-GSPT2 antibody (182-232 aa) (STJ197719)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human ERF3B at the amino acid range 182-232
General Information
| Short Description | Rabbit polyclonal anti-Eukaryotic peptide chain release factor GTP-binding subunit ERF3B (182-232 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSPT2 |
| Gene ID | 23708 |
| Uniprot ID | ERF3B_HUMAN |
| Immunogen | Synthesized peptide derived from the human ERF3B at the amino acid range 182-232 |
| Immunogen Region | 182-232 aa |
| Specificity | This antibody detects endogenous levels of human ERF3B |
Additional Info
| Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT2/ERF3B mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT2/ERF3B induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. |
| Protein Name | Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3bEukaryotic Peptide Chain Release Factor Subunit 3bErf3bG1 To S Phase Transition Protein 2 Homolog |
| Database Links | Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3b antibodyAnti-Eukaryotic Peptide Chain Release Factor Subunit 3b antibodyAnti-Erf3b antibodyAnti-G1 To S Phase Transition Protein 2 Homolog antibodyAnti-GSPT2 antibodyAnti-ERF3B antibody |
Information sourced from Uniprot.org