Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A.X Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-H2AX antibody (94-143 aa) (STJ93520)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Histone H2A.X at the amino acid range 94-143
STJ93520
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General Information

Short DescriptionRabbit polyclonal anti-Histone H2AX (94-143 aa) for use in WB, IHC, IF, CoIP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/CoIP/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
IP 1:200-500
ELISA 1:10000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolH2AX
Gene ID3014
Uniprot IDH2AX_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Histone H2A.X at the amino acid range 94-143
Immunogen Region94-143 aa
SpecificityHistone H2A.X Polyclonal Antibody detects endogenous levels of Histone H2A.X protein.

Additional Info

Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Protein Name Histone H2ax
H2a/X
Histone H2a.x
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214858
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-68616
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-9710421
Reactome: R-HSA-977225
Reactome: R-HSA-9821002
Reactome: R-HSA-9821993
Reactome: R-HSA-9841922
Reactome: R-HSA-9843940
Reactome: R-HSA-9843970
Reactome: R-HSA-9845323
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H2ax antibody
Anti-H2a/X antibody
Anti-Histone H2a.x antibody
Anti-H2AX antibody
Anti-H2AFX antibody

Information sourced from Uniprot.org

Citations

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