Anti-GSPT1 antibody (138-420) (STJ118654)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GSPT1 (138-420) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSPT1 |
| Gene ID | 2935 |
| Uniprot ID | ERF3A_HUMAN |
| Immunogen Region | 138-420 |
| Immunogen Sequence | SMELSEPIENGETEMSPEES WEHKEEISEAEPGGGSLGDG RPPEESAHEMMEEEEEIPKP KSVVAPPGAPKKEHVNVVFI GHVDAGKSTIGGQIMYLTGM VDKRTLEKYEREAKEKNRET WYLSWALDTNQEERDKGKTV EVGRAYFETEKKHFTILDAP GHKSFVPNMIGGASQADLAV LVISARKGEFETGFEKGGQT REHAMLAKTAGVKHLIVLIN KMDDPTVNWSNERYEECKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 138-420 of human GSPT1 (NP_001123478.1). |
Additional Info
| Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. |
| Protein Name | Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3aEukaryotic Peptide Chain Release Factor Subunit 3aErf3aG1 To S Phase Transition Protein 1 Homolog |
| Database Links | Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a antibodyAnti-Eukaryotic Peptide Chain Release Factor Subunit 3a antibodyAnti-Erf3a antibodyAnti-G1 To S Phase Transition Protein 1 Homolog antibodyAnti-GSPT1 antibodyAnti-ERF3A antibody |
Information sourced from Uniprot.org