| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GSPT1 (138-420) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GSPT1 |
| Gene ID: |
2935 |
| Uniprot ID: |
ERF3A_HUMAN |
| Immunogen Region: |
138-420 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 138-420 of human GSPT1 (NP_001123478.1). |
| Immunogen Sequence: |
SMELSEPIENGETEMSPEES WEHKEEISEAEPGGGSLGDG RPPEESAHEMMEEEEEIPKP KSVVAPPGAPKKEHVNVVFI GHVDAGKSTIGGQIMYLTGM VDKRTLEKYEREAKEKNRET WYLSWALDTNQEERDKGKTV EVGRAYFETEKKHFTILDAP GHKSFVPNMIGGASQADLAV LVISARKGEFETGFEKGGQT REHAMLAKTAGVKHLIVLIN KMDDPTVNWSNERYEECKE |
| Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. |
| Protein Name | Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3aEukaryotic Peptide Chain Release Factor Subunit 3aErf3aG1 To S Phase Transition Protein 1 Homolog |
| Database Links | Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Alternative Antibody Names | Anti-Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a antibodyAnti-Eukaryotic Peptide Chain Release Factor Subunit 3a antibodyAnti-Erf3a antibodyAnti-G1 To S Phase Transition Protein 1 Homolog antibodyAnti-GSPT1 antibodyAnti-ERF3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
