Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using Phospho-Smad3-T179 Rabbit pAb (STJ114841). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SMAD3-T179 antibody (STJ114841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-Smad3-T179 antibody [1A1] (STJA0019135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$510.98 - $2,203.88

Choose Options

Western blot analysis of HELA cells using Phospho-Smad3 (T179) Polyclonal Antibody

Anti-Phospho-SMAD3-Thr179 antibody (145-194 aa) (STJ90905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SMAD4-Thr276 antibody (STJA0009889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SMAD5-S463/465 antibody [S5792RM] (STJ11105792)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-Smad5-S463/S465 Rabbit polyclonal antibody (STJ11105818) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-SMAD5-S463/S465 antibody (STJ11105818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb (STJ11102605) at 1:1000 dilution or Smad5 antibody (STJ25609). Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SMAD5-S463/S465 antibody [S5MR] (STJ11102605)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

Choose Options

Western blot analysis of C6, using Phospho-Smad5-S465 Rabbit monoclonal antibody (STJ11105872) at 1:2000 dilution. C6 cells were treated by MG132 (50 Mu M) and TGF Beta at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-SMAD5-S465 antibody [S5872RM] (STJ11105872)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$197.78 - $867.38

Choose Options

Anti-Phospho-SMAD5-Ser463/465 antibody [PT0663R] (STJA0039233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

Add to Cart The item has been added

Anti-Phospho-SMAD9-Y92 antibody [S5897RM] (STJ11105897)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

Choose Options

Western blot analysis of various lysates, using Phospho-SMC1-S957 Rabbit pAb (STJ22400) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-SMC1A-S957 antibody (STJ22400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb (STJ11102639) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-SMC1A-S957 antibody [S9MR] (STJ11102639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMC1A-Ser360 antibody (STJ196431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SMC1 (Phospho-Ser957) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SMC1A-Ser957 antibody (931-980 aa) (STJ90406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Western blot analysis of K562 using p-SMC1 (S966) antibody.

Anti-Phospho-SMC1A-Ser966 antibody (932-981 aa) (STJ90831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMC3-Ser1083 antibody (STJ193208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SMO-S611 antibody (STJ11107677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from Jurkat cells, using Phospho-SMO-S611/615 Rabbit pAb (STJ11101073) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Jurkat cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 20 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SMO-S611/615 antibody (STJ11101073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-SMO-S611/615/616 Rabbit pAb (STJ11101074) at 1:1000 dilution. 293T-SMO cells were treated by SHH (0. 4 Mu g/mL) at 37 °C after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1min.

Anti-Phospho-SMO-S611/615/616 antibody (STJ11101074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Anti-Phospho-SMO-S615 antibody (STJ11101071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Anti-Phospho-SMO-S615/616 antibody (STJ11107678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Dot-blot analysis of all sorts of peptides using Phospho-SMO-S616 antibody (STJ11101072) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-SMO-S616 antibody (STJ11101072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of HT29 COS7 cells using Phospho-SNAI 1 (S246) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-SNAI1-Ser246 antibody (215-264 aa) (STJ90728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

Choose Options

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 400 units/100ul lysate for 30 minutes, RT).

Anti-Phospho-SNAP25-Ser187 antibody (STJA0003778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Guinea Pig/Human/Non-Human Primates/Zebrafish

$613.58

Choose Options

Western blot analysis of lysates from mouse brain tissue, using Phospho-Alpha-Synuclein-S129 Rabbit pAb (STJ22404). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SNCA-S129 antibody (STJ22404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Immunohistochemistry analysis of paraffin-embedded human brain, using Synuclein (Phospho-Ser129) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SNCA-Ser129 antibody (91-140 aa) (STJ90418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot of rat cortical lysate showing specific immunolabeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-SNCA-Ser129 antibody (STJA0003577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Non-Human Primates

$613.58

Choose Options

Western blot analysis of various cells using Phospho-Synuclein-Alpha (Y125) Polyclonal Antibody

Anti-Phospho-SNCA-Tyr125 antibody (91-140 aa) (STJ91284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from 293 cells treated with Etoposide 25 muM 60', using Synuclein-alpha (Phospho-Tyr133) Antibody. The lane on the left is blocked with the phospho peptide.

Anti-Phospho-SNCA-Tyr133 antibody (91-140 aa) (STJ90680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from mouse brain, using Synuclein-alpha (Phospho-Tyr136) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SNCA-Tyr136 antibody (91-140 aa) (STJ90681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of extracts of various cell lines, using Phospho-Alpha-Synuclein-Y133 polyclonal antibody (STJ11101127) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-SNCA-Y133 antibody (STJ11101127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

Choose Options

Western blot analysis of various lysates, using Phospho-Alpha-Synuclein-Y133 pAb (STJ11105783) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SNCA-Y133 antibody (STJ11105783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from Mouse brain, using Phospho-Alpha-Synuclein-Y136 Rabbit pAb (STJ22403) at 1:1000 dilution. Mouse brain were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SNCA-Y136 antibody (STJ22403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from recombinant Phospho-SnRK2. 6-S175 Protein using Phospho-SnRK2. 6-S175 pAb (STJ11107728) at 1:500 dilution incubated overnight at 4°C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 100 ng per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( 5 s.

Anti-Phospho-SnRK2.6-S175 antibody (STJ11107728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

$197.78 - $867.38

Choose Options

Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-SOX2-Ser250/Ser251 antibody (STJ194747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Anti-Phospho-SOX2-Thr-118 antibody (STJA0005590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$496.13

Choose Options

Anti-Phospho-SOX2-Thr-128 antibody (STJA0005592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$496.13

Choose Options

Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-SOX9-Ser181 antibody (147-196 aa) (STJ91055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from 3T3 cells, using Phospho-SP1-T739 Rabbit pAb (STJ22406). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SP1-T739 antibody (STJ22406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SP1-Thr453 antibody (421-470 aa) (STJ90407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of various cells using Phospho-Sp1 (T739) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SP1-Thr739 antibody (706-755 aa) (STJ90408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

Choose Options

Anti-Phospho-SPHK1-Ser-225 antibody (STJA0005597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$496.13

Choose Options

Western Blot analysis of 1 MCF-7 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SPHK1-Ser225 antibody (STJA0009903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SPHK2-Thr-578 antibody (STJA0005600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$496.13

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SPHK2 (Phospho-Thr614) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SPHK2-Thr614 antibody (580-629 aa) (STJ91268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Ser349 antibody (STJ196432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Ser403 antibody (STJ196433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Thr269/Ser272 antibody (STJ196434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options