• Western blot analysis of lysates from K562 cells, using Ret (Phospho-Tyr1062) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of Ret (phospho Tyr1062) Polyclonal Antibody, using HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody (cat: (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-RET-Tyr1062 antibody (1041-1090 aa) (STJ90679)

SKU:
STJ90679

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Proto-oncogene tyrosine-protein kinase receptor Ret-Tyr1062 (1041-1090 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RET
Gene ID: 5979
Uniprot ID: RET_HUMAN
Immunogen Region: 1041-1090 aa
Specificity: Phospho-Ret (Y1062) Polyclonal Antibody detects endogenous levels of Ret protein only when phosphorylated at Y1062.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Ret around the phosphorylation site of Tyr1062 at the amino acid range 1041-1090
Function Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis.involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor.in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers.these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.
Protein Name Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret
Cadherin Family Member 12
Proto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment
Database Links Reactome: R-HSA-5673001
Reactome: R-HSA-8853659
Reactome: R-HSA-9768919
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Predominantly Located On The Plasma Membrane
In The Presence Of Sorl1 And Gfra1
Directed To Endosomes
Alternative Antibody Names Anti-Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret antibody
Anti-Cadherin Family Member 12 antibody
Anti-Proto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment antibody
Anti-RET antibody
Anti-CDHF12 antibody
Anti-CDHR16 antibody
Anti-PTC antibody
Anti-RET51 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance