Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from Jurkat cells using Phospho-Syk-Y323 Rabbit pAb (STJ22425). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYK-Y323 antibody (STJ22425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y525/526 Rabbit pAb (STJ113492) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Syk-Y525/526 antibody (STJ113492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (STJ113483) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYK/ZAP70-Y319 antibody (STJ113483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from mouse Brain tissue using Phospho-SYN1-S9 Rabbit pAb (STJ22426). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SYN1-S9 antibody (STJ22426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-SYN1-Ser549 antibody (STJA0003785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Rat/Bovine/Canine/Human/Non-Human Primates

£454.50

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Western blot analysis of 293T using SYN1 p-S553 antibody. Antibody was diluted at 1:500

Anti-Phospho-SYN1-Ser553 antibody (STJ97692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-SYN1-Ser603 antibody (STJA0003786)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Xenopus/Zebrafish

£454.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using Synapsin1 (Phospho-Ser605) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SYN1-Ser605 antibody (576-625 aa) (STJ91309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells treated with Anisomycin 25ug/ml 30', using Synapsin1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SYN1-Ser62 antibody (26-75 aa) (STJ90832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62, 67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-SYN1-Ser62/67 antibody (STJA0003784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine

£454.50

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Western blot analysis of various cells using Phospho-Synapsin I (S9) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SYN1-Ser9 antibody (3-52 aa) (STJ90417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9 (STJA0003783, 1:500, red). Cells and photo courtesy of QBMCellScience.

Anti-Phospho-SYN1-Ser9 antibody (STJA0003783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Rat/Zebrafish/Bovine/Human/Xenopus

£454.50

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Western blot analysis of various cells using Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody

Anti-Phospho-Synaptotagmin 1/2-Ser309/306 antibody (276-325 aa) (STJ90746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Synaptotagmin 1/2 (T202/199) Polyclonal Antibody

Anti-Phospho-Synaptotagmin 1/2-Thr202/199 antibody (176-225 aa) (STJ90747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.

Anti-Phospho-SYT1-Ser309 antibody (STJA0003792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Zebrafish

£454.50

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Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (STJA0003791, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at-20C for 10 minutes. Photo courtesy of Gang Liu.

Anti-Phospho-SYT1-Thr202 antibody (STJA0003791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Zebrafish

£454.50

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Anti-Phospho-TAB2-Ser372 antibody (STJ196444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-TACC3-Ser558 antibody (STJ196445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using (STJ11103659) at 1:1000 dilution. C2C12 cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-TAK1-S439 antibody [S9MR] (STJ11103659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Anti-Phospho-TAK1-T184 antibody (STJ11107722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using TAL-1 (Phospho-Ser122) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TAL1-Ser122 antibody (96-145 aa) (STJ90638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at Ser181 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-TAOK2-Ser181 antibody (STJA0003794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Canine/Chicken/Finch/Xenopus/Zebrafish

£454.50

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Western blot analysis of various lysates, using Phospho-Tau-T181 Rabbit pAb (STJ11103760) at 1:600 dilution. Mouse brain cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-Tau-T181 antibody (STJ11103760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-Tau-T217 Rabbit pAb (STJ11103759) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-Tau-T217 antibody (STJ11103759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of recombinant Human p-Tau217 Protein using Phospho-Tau-T217 Rabbit mAb (STJ11105851) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 100ng Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 5s.

Anti-Phospho-Tau-T217 antibody [S5851RM] (STJ11105851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-TBC1D4-S588 Rabbit pAb (STJ117888) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-TBC1D4-S588 antibody (STJ117888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13 (1) :1-15. Human, mouse 1:1200 liver tissue hUC-MSC

Anti-Phospho-TBC1D4-Ser318 antibody (STJ196242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-TBC1D4-Ser588 antibody (STJ196243)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, using Phospho-TBC1D4-T642 Rabbit pAb (STJ117889) at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-TBC1D4-T642 antibody (STJ117889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of L929 using p-TBC1D4 (T642) antibody. Antibody was diluted at 1:2000

Anti-Phospho-TBC1D4-Thr642 antibody (611-660 aa) (STJ91288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-TBK1/NAK-S172 Rabbit pAb (STJ11100988) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-TBK1-S172 antibody (STJ11100988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-TBK1/NAK-S172 Rabbit monoclonal antibody (STJ11105874) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-TBK1-S172 antibody [S5874RM] (STJ11105874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-TBK1/NAK-S172 Rabbit mAb (STJ11102607) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-TBK1-S172 antibody [S7MR] (STJ11102607)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Manganese ion gradient liposomes modified by disulfide bond enhance cellular uptake and tumor immunity. Haiyang Hu WB mouse B16F10 cell-xenograft, 4T1 cell-xenograft

Anti-Phospho-TBK1-Ser172 antibody (STJ196450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using E2A (Phospho-Thr355) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TCF3-Thr355 antibody (321-370 aa) (STJ91252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Dot-blot analysis of all sorts of peptides using Phospho-TDP-43/TARDB-S409/S410 antibody (STJ11103763) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-TDP-43/TARDB-S409/S410 antibody (STJ11103763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-TEK-Ser1119 antibody (STJ196451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using TIE2 (Phospho-Tyr1102) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TEK-Tyr1102 antibody (1068-1117 aa) (STJ91136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TEK-Tyr1108 antibody (1074-1123 aa) (STJ90693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody

Anti-Phospho-TEK-Tyr992 antibody (STJ90597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-TRF1 (S219) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-TERF1-Ser219 antibody (185-234 aa) (STJ90560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TERF2-Ser365 antibody (STJA0009967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-TERF2-Thr188 antibody (STJA0009965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Telomerase (Phospho-Ser227) Antibody. The lane on the right is blocked with the Telomerase (Phospho-Ser227) peptide.

Anti-Phospho-TERT-Ser227 antibody (196-245 aa) (STJ91354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from COLO205 cells, using Telomerase (Phospho-Ser824) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TERT-Ser824 antibody (796-845 aa) (STJ90548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-TET2-Ser99 antibody (STJ11107684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-CD71 (S24) Polyclonal Antibody

Anti-Phospho-TFRC-Ser24 antibody (15-64 aa) (STJ90889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western Blot analysis of 1 A431 cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TGFB1I1-Tyr60 antibody (STJA0009949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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