Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from NIH/3T3 cells using Phospho-FOXO3A-S253 Rabbit pAb (STJ114850) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-FOXO3-S253 antibody (STJ114850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO3A-S294 Rabbit pAb (STJ11100997) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-FOXO3-S294 antibody (STJ11100997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-FOXO3-Ser253 antibody (220-269 aa) (STJ90275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Zebrafish

£49.00 - £261.00

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Western blot analysis of Phospho-FOXO3A (Ser253) in 3T3 lysates using Phospho-FOXO3A (Ser253) antibody.

Anti-Phospho-FOXO3-Ser253 antibody (248-260 aa) [R06-2G6] (STJA0011330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-FOXO3-Ser294 antibody (STJ196308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-FOXO3-Ser315 antibody (STJA0009840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FOXO3-Ser318 antibody (STJA0010058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-FOXO3-Ser318/321 antibody (STJ196309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FOXO3-Ser321 antibody (STJA0010059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-FOXO3-Ser413 antibody (STJ196310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-FOXO3-Ser425 antibody (STJ196311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-FOXO3-Ser644 antibody (STJA0010017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-FOXO3-Ser7 antibody (STJ196312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K-562 cells, using Phospho-FOXO3A-S425 Rabbit pAb (STJ11103738) at 1:1000 dilution. K-562 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-FOXO3A-S425 antibody (STJ11103738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-FOXO3A-Ser253 antibody [PT0061R] (STJA0039645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-FOXO3A-Ser253 antibody [R08-7U1] (STJA0035964)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates, using Phospho-FOXO4-S197 Rabbit pAb (STJ22147) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-FOXO4-S197 antibody (STJ22147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-FOXO4-Ser193 antibody (STJ196313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-FoxO4 (S197) Polyclonal Antibody

Anti-Phospho-FOXO4-Ser197 antibody (164-213 aa) (STJ90276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells treated with starved 24h, using FOXO4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FOXO4-Ser262 antibody (228-277 aa) (STJ90930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 5', using FOXO4 (Phospho-Thr451) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FOXO4-Thr451 antibody (417-466 aa) (STJ90542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FRS2-Tyr196 antibody (STJ196314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-FRS2 (Y436) Polyclonal Antibody

Anti-Phospho-FRS2-Tyr436 antibody (402-451 aa) (STJ90965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Anti-Phospho-FSCN1-Ser-39 antibody (STJA0005420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Anti-Phospho-FSCN1-Ser-39 antibody [M315] (STJA0005419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb (STJ110850) at 1:1000 dilution. A-431 cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-FYN-S21 antibody (STJ110850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of LOVO+UV cells using Phospho-Fyn (Y530) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-FYN-Tyr530 antibody (488-537 aa) (STJ90792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293 cells, using Phospho-G3BP1-S232 Rabbit pAb (STJ22148). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-G3BP1-S232 antibody (STJ22148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£126.50 - £618.50

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Western Blot analysis of HeLa mouse brain tissue using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-G3BP1-Ser149 antibody (STJA0010081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-G3BP1 (S232) Polyclonal Antibody

Anti-Phospho-G3BP1-Ser232 antibody (216-248 aa) (STJ90277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-GAB1-Tyr307 antibody (STJ91230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Gab 1 (Y627) Polyclonal Antibody

Anti-Phospho-GAB1-Tyr627 antibody (611-643 aa) (STJ90278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-Gab 1 (Y659) Polyclonal Antibody

Anti-Phospho-GAB1-Tyr659 antibody (644-674 aa) (STJ91067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-GAB1-Y307 Rabbit polyclonal antibody (STJ11105790) at 1:1000 dilution. NIH/3T3 cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-GAB1-Y307 antibody (STJ11105790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GAB1-Y659 Rabbit pAb (STJ116394) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-GAB1-Y659 antibody (STJ116394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using GRB2 (Phospho-Ser159) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-GAB2-Ser159 antibody (125-174 aa) (STJ90967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Gab2 (Phospho-Ser623) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GAB2-Ser623 antibody (589-638 aa) (STJ90978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-GAB2-Tyr452 antibody (STJ90630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 HeLa cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-GAB2-Tyr614 antibody (STJA0010078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using Gab2 (Phospho-Tyr643) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GAB2-Tyr643 antibody (609-658 aa) (STJ90977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R1 protein phosphorylated at Ser923 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-GABBR1-Ser923 antibody (STJA0003653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Human/Non-Human Primates

£488.50

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Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R2 protein phosphorylated at Ser783 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-GABBR2-Ser783 antibody (STJA0003652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Xenopus

£488.50

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Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-GABBR2-Ser893 antibody (STJA0009854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-GABAA R Beta 1 (S434) Polyclonal Antibody

Anti-Phospho-GABRB1-Ser434 antibody (401-450 aa) (STJ90736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA Beta 3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-GABRB3-Ser408/409 antibody (STJA0003648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Xenopus/Zebrafish

£488.50

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Western blot of rat cortical lysate showing specific immunolabeling of the ~45 kDa GABAA Gamma 2 protein phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-GABRG2-Ser327 antibody (STJA0003651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates

£488.50

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Anti-Phospho-Gamma-Catenin-Tyr-550 antibody (STJA0041138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-Gamma-H2AX-S139 antibody [9F3] (STJA0019679)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

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