• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-GAB1-Y659 antibody (STJ116394) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GAB1-Y659 Rabbit polyclonal antibody (STJ116394) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-GAB1-Y659 antibody (STJ116394)

SKU:
STJ116394

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-GAB1-Y659 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAB1
Gene ID: 2549
Uniprot ID: GAB1_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y659 of human GAB1 (NP_002030.2).
Immunogen Sequence: VDYVV
Post Translational Modifications Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Function Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.
Protein Name Grb2-Associated-Binding Protein 1
Grb2-Associated Binder 1
Growth Factor Receptor Bound Protein 2-Associated Protein 1
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1236382
Reactome: R-HSA-1257604
Reactome: R-HSA-180292
Reactome: R-HSA-1963642
Reactome: R-HSA-2219530
Reactome: R-HSA-5637810
Reactome: R-HSA-5654689
Reactome: R-HSA-5654695
Reactome: R-HSA-5654710
Reactome: R-HSA-5654720
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-6811558
Reactome: R-HSA-8851907
Reactome: R-HSA-8853659
Reactome: R-HSA-8865999
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-9027276
Reactome: R-HSA-9028335
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Alternative Antibody Names Anti-Grb2-Associated-Binding Protein 1 antibody
Anti-Grb2-Associated Binder 1 antibody
Anti-Growth Factor Receptor Bound Protein 2-Associated Protein 1 antibody
Anti-GAB1 antibody

Information sourced from Uniprot.org

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