Post Translational Modifications | Primary antibodies

Western blot analysis of extracts from Hela cells, 1:2000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H4-Arg3 antibody (STJ97197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ11103476) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Mono-Methyl-Histone H4-K20 antibody (STJ11103476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23970). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-K20 antibody (STJ23970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H4-K5 Rabbit polyclonal antibody (STJ119872) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-K5 antibody (STJ119872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of 3T3 mouse-kidney KB K562 Hela 293T lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-Histone H4-Lys21 antibody (21 aa) (STJ98696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H4-Lys79 antibody (STJ97157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ11100898). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H4-R3 antibody (STJ11100898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of HeLa cells using MonoMethyl-NONO-R357/R365 polyclonal antibody (STJ110387) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-NONO-R357/R365 antibody (STJ110387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of MCF7 cells using Mono-Methyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-RELA-Lys314/Lys315 antibody (STJ90114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T COLO using TP53 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Mono-Methyl-TP53-Lys370 antibody (STJ97694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using P53 (Mono Methyl Lys370) Mouse mAb diluted at 1:500.

Anti-Mono-Methyl-TP53-Q59 antibody [Q59] (STJ97495)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody (STJ118448) at 1:1000 dilution.

Anti-Mono-Methyl-UHRF1-K385 antibody (STJ118448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using MonoMethyl-YAP1-K342 Rabbit polyclonal antibody (STJ11100581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Mono-Methyl-YAP1-K342 antibody (STJ11100581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of MonoMethyl-Histone H2B-K116 in paraffin-embedded rat heart using MonoMethyl-Histone H2B-K116 Rabbit monoclonal antibody (STJ11103791) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MonoMethyl-Histone H2B-K116 antibody [S3791RM] (STJ11103791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Anti-MonoMethyl-Histone H4-R3 antibody (STJ11106422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-MonoMethyl-PTEN-K349 antibody (STJ11106694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-MonoMethyl-PTEN-K349 antibody (STJ11106739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Pan-Acetyl-ACTA2-Lys52/50/51 antibody (STJA0005951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Pan-Acetyl-ACTA2-Lys63/61/62 antibody (STJA0006005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Pan-Acetyl-KRT2-Lys185 antibody (STJA0005971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Pan-Acetyl-MYH1-Lys1394 antibody (STJA0005982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Pan-Acetyl-MYH1-Lys505 antibody (STJA0005983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela using Pan Methylated Lysine monoclonal antibody.

Anti-Pan-Methyl-Lysine-Mix antibody [Mix] (STJ97446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Species independent

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Phospho-PKC (pan) (gamma) -T514 antibody (STJ29379) at 1:1000 dilution. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Pan-Phospho-PKC Gamma-T514 antibody (STJ29379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-14-3-3 Beta/Zeta-Ser184/186 antibody (151-200 aa) (STJ90562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58 (STJA0003567, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.

Anti-Phospho-14-3-3-Ser58 antibody (STJA0003567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Sheep/Xenopus/Zebrafish

£454.50

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-4E-BP1-Thr69 antibody (35-84 aa) (STJ90163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Thr735 antibody (701-750 aa) (STJ90579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-ABL1-Thr735 antibody (STJ196256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-ABL1-Tyr-245 antibody (STJA0005239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-ABL1-Tyr-412 antibody (STJA0005240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ABL1-Tyr204 antibody (156-205 aa) (STJ90452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Tyr245 antibody (196-245 aa) (STJ90694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-ABL1-Tyr412 antibody (STJ196258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ABL1-Tyr89 antibody (STJ196259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-ABL1-Y204 antibody (STJ22002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of C6 cells, using Phospho-ABL1-Y245 polyclonal antibody (STJ22000) at 1:1000 dilution or ABL1 antibody (A0282). C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3S.

Anti-Phospho-ABL1-Y245 antibody (STJ22000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of C6 cells, using Phospho-ABL1-Y412 polyclonal antibody (STJ11101098) at 1:1000 dilution or ABL1 antibody (A0282). C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-ABL1-Y412 antibody (STJ11101098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-ABL1-Y412 antibody (STJ22003). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-ABL1-Y412 antibody (STJ22003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Abl (Phospho-Tyr412) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Abl1/2-Tyr393/439 antibody (361-410 aa) (STJ90779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using Abl (Phospho-Tyr393/412) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Abl1/2-Tyr393/439 antibody (406-455 aa) (STJ90164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of A-431, using Phospho-ACC1-S79 rabbit polyclonal antibody (STJ22006) at 1:700 dilution. A-431 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-ACACA-S79 antibody (STJ22006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-ACC Alpha (S80) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ACACA-Ser80 antibody (46-95 aa) (STJ90755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 3T3 using p-ACC Alpha (S80) antibody. Antibody was diluted at 1:1000

Anti-Phospho-ACACA-Ser80 antibody (46-95 aa) (STJ90780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-ACAP1-Ser554 antibody (520-569 aa) (STJ91177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-ACC1/ACC2-S79 antibody [SRM] (STJ11107725)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of NIH/3T3, using Phospho-Acetyl CoA Carboxylase-S79 Rabbit polyclonal antibody (STJ11105871) at 1:1000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-Acetyl CoA Carboxylase-S79 antibody (STJ11105871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £642.50

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Western blot analysis of NIH/3T3, using Phospho-Acetyl CoA Carboxylase-S79 antibody (STJ11105862) at 1:7000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-Acetyl CoA Carboxylase-S79 antibody [S5862RM] (STJ11105862)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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