Western Blot Antibodies | St John's Laboratory

Western blot analysis of 1) Mouse skeletal muscle tissue, 2) Mouse kidney tissue, 3) Mouse Heart Tissue, 4) Mouse brain tissue, 5) Mouse Liver tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Alpha Tubulin antibody (STJ197321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of a-Tubulin at dilution of 1:2000

Anti-alpha Tubulin antibody [SM5232] (STJA0005232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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STJ73368 (0. 3µg/ml) staining of albumin-depleted Serum (A) and Plasma (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antichymotrypsin antibody (222-236) (STJ73368)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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STJ73055 (0. 01µg/ml) staining of Albumin-depleted Plasma (A) and Serum (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antitrypsin antibody (230-241) (STJ73055)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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STJ73054 (0. 3µg/ml) staining of Albumin-depleted Human Plasma lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antitrypsin antibody (Internal) (STJ73054)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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STJ73369 (0. 5µg/ml) staining of Human Albumin-depleted Serum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-alpha-2-macroglobulin antibody (Internal) (STJ73369)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Anti-Alpha-2A adrenergic receptor antibody [N379/52] (STJA0020734)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-Alpha-2A adrenergic receptor antibody [N379/72] (STJA0020735)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-Alpha-2A adrenergic receptor antibody [N379/79] (STJA0020736)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-Alpha-2C adrenergic receptor antibody [N330A/51] (STJA0020777)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-Alpha-2C adrenergic receptor antibody [N330A/80] (STJA0020778)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-Alpha-2C adrenergic receptor antibody [N378/107] (STJA0020779)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-Alpha-Actinin antibody [CB11] (STJ16100814)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ELISA

Reactivity :

Human/Chicken

£567.50

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STJ73360 (0. 3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-fetoprotein antibody (68-82) (STJ73360)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Anti-Alpha-Fetoprotein antibody [AFP-01] (STJ16100608)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/ELISA/WB

Reactivity :

Human

£350.50

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Anti-Alpha-Fodrin antibody [AA6] (STJ16100815)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ELISA

Reactivity :

Human/Rat/Chicken/Rabbit/Guinea Pig

£685.50

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Anti-Alpha-Gal Epitope antibody [B1H12] (STJA0019089)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FuncS/IHC-P/WB

Reactivity :

Mouse/Rat/Porcine

£378.50 - £1,632.50

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Anti-Alpha-Gal Epitope antibody [B4H2] (STJA0019090)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FuncS/IHC-P/WB

Reactivity :

Mouse/Rat/Porcine

£378.50 - £1,632.50

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Anti-Alpha-Gal Epitope antibody [Gal13] (STJA0019091)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FuncS/IHC-P/WB

Reactivity :

Mouse/Rat/Porcine

£378.50 - £1,632.50

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Anti-Alpha-Gal Epitope antibody [M86] (STJA0019083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FuncS/IHC-P/WB

Reactivity :

Mouse/Rat/Porcine

£378.50 - £1,632.50

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Anti-Alpha-Gal Epitope antibody [M86] (STJA0019088)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FuncS/IHC-P/WB

Reactivity :

Mouse/Rat/Porcine

£378.50 - £1,632.50

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Anti-Alpha-IIb-Integrin antibody [CA3] (STJ16100813)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ELISA

Reactivity :

Human

£606.50

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Western blot analysis of various lysates using Alpha-synuclein Mouse mAb (STJ11107379) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-Alpha-synuclein antibody (1-140 aa) [SMM] (STJ11107379)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Alpha-Synuclein antibody (59-140 aa) [SRM] (STJ11106873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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STJ72875 (0. 1µg/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha-synuclein antibody (Internal) (STJ72875)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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STJ72876 (0. 1µg/ml) staining of Human Amygdala lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha-synuclein antibody (Internal) (STJ72876)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Pig

£312.50

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Anti-Alpha-Synuclein antibody [AS11] (STJ16102412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC-F

Reactivity :

Human

£636.50

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Anti-Alpha-Synuclein antibody [AS11] {Alexa-fluor-488} (STJ16102409)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC-F

Reactivity :

Human

£770.50

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Anti-Alpha-Synuclein antibody [AS11] {Alexa-fluor-546} (STJ16102410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC-F

Reactivity :

Human

£770.50

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Anti-Alpha-Synuclein antibody [AS11] {Alexa-fluor-647} (STJ16102411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC-F

Reactivity :

Human

£770.50

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Anti-Alpha-Synuclein antibody [AS11] {HRPO} (STJ16102659)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC-F

Reactivity :

Human

£712.50

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Anti-Alpha-synuclein antibody [N335/10] (STJA0021571)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Western blot analysis of various lysates using Alpha-Tubulin Rabbit pAb (STJ11103685) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£160.50 - £300.50

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Western blot analysis of extracts of various cell lines, using Alpha-Tubulin antibody (STJ11103692) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£160.50 - £300.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103689) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£96.50 - £392.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103690) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£207.50 - £392.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit mAb (STJ11103694) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) [S4MR] (STJ11103694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £662.50

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Immunofluorescence analysis of Hela cell. 1, DAPK3 (phospho Thr265) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Alpha-tubulin antibody (350-451 aa) [8F11] (STJ96937)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse/D.melanogaster

£48.00 - £252.00

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Western blot analysis of C. Elegans whole body lysate using mouse monoclonal antibody diluted at 1:20,000.

Anti-Alpha-tubulin antibody [Mix] (STJ196642)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

C.elegans/Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates using Alpha-Tubulin Mouse mAb (STJ11107556) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Alpha-Tubulin antibody [SMM] (STJ11107556)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£64.50 - £300.50

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Anti-Alpha-Tubulin antibody [TU-01] (STJ16100473)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IP/FC

Reactivity :

Mouse/Pig/Human/Turkey/Eisenia/Paramecium/Nicotiana/Yeast/Arabidopsis

£485.50

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Anti-Alpha-zein 19KD antibody (STJ11106680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Z.mays

£146.50 - £642.50

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STJ71657 (0. 1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Alpha2-antiplasmin antibody (Internal) (STJ71657)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of 293F-ALPI, using ALPI rabbit polyclonal antibody (STJ27982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALPI antibody (20-527) (STJ27982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALPK1 using ALPK1 Rabbit pAb (STJ11106978) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s. <br><b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>

Anti-ALPK1 antibody (1-473 aa) (STJ11106978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using Alpha-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ALPK1 antibody (11-60 aa) (STJ96366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ALPK1 Rabbit polyclonal antibody (STJ11103266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALPK1 antibody (340-500) (STJ11103266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ALPK3 antibody (730-810 aa) (STJ190245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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