Anti-ALDH1A1 antibody (21-70 aa) (STJ96522)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human ALDH1A1 at the amino acid range 21-70
General Information
| Short Description | Rabbit polyclonal anti-Aldehyde dehydrogenase 1A1 (21-70 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALDH1A1 |
| Gene ID | 216 |
| Uniprot ID | AL1A1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human ALDH1A1 at the amino acid range 21-70 |
| Immunogen Region | 21-70 aa |
| Specificity | ALDH1A1 Polyclonal Antibody detects endogenous levels of ALDH1A1 protein. |
Additional Info
| Post Translational Modifications | The N-terminus is blocked most probably by acetylation. |
| Function | Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic. Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification. Also functions downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins. Also has an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission. |
| Protein Name | Aldehyde Dehydrogenase 1a1 3-Deoxyglucosone DehydrogenaseAldh-E1AlhdiiAldehyde Dehydrogenase Family 1 Member A1Aldehyde Dehydrogenase - CytosolicRetinal Dehydrogenase 1Raldh 1Raldh1 |
| Database Links | Reactome: R-HSA-5365859Reactome: R-HSA-70350Reactome: R-HSA-71384 |
| Cellular Localisation | CytoplasmCytosolCell ProjectionAxon |
| Alternative Antibody Names | Anti-Aldehyde Dehydrogenase 1a1 antibodyAnti-3-Deoxyglucosone Dehydrogenase antibodyAnti-Aldh-E1 antibodyAnti-Alhdii antibodyAnti-Aldehyde Dehydrogenase Family 1 Member A1 antibodyAnti-Aldehyde Dehydrogenase - Cytosolic antibodyAnti-Retinal Dehydrogenase 1 antibodyAnti-Raldh 1 antibodyAnti-Raldh1 antibodyAnti-ALDH1A1 antibodyAnti-ALDC antibodyAnti-ALDH1 antibodyAnti-PUMB1 antibody |
Information sourced from Uniprot.org