Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALKBH5 antibody (STJ193198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ALKBH5 antibody [PT1017R] (STJA0039943)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-ALKBH6 antibody (17-67) (STJA0006571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ALKBH6 Rabbit pAb (STJ112044) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALKBH6 antibody (97-266) (STJ112044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ALKBH7 antibody (STJ22590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALKBH7 antibody (1-221) (STJ22590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALKBH7 antibody (10-90 aa) (STJ190427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using ALKBH8 antibody (STJ29222) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ALKBH8 antibody (1-230) (STJ29222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ALKBH8 antibody (27-77) (STJA0006515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Allergen Dog 1 antibody [SAA0620] (STJA0019441)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Dog

£378.50 - £1,632.50

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Anti-Allergen Pis v 2/11S globulin antibody [SAA0756] (STJA0019575)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/IP/WB

Reactivity :

P.vera

£378.50 - £1,632.50

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Western blot analysis of purified alliinase, diluted at 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Alliinase antibody (STJ197444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

A.sativum L

£48.00 - £252.00

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Western blot analysis of various cells using ALMS1 Polyclonal Antibody

Anti-ALMS1 antibody (1530-1610 aa) (STJ91564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ALMS1 antibody (3223-3250 aa) (STJ13100559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£331.50

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Anti-ALMS1 antibody (4100-4150 aa) (STJ13100616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-ALMS1P antibody (127-177) (STJA0006369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of A-431, using ALOX12 antibody (STJ116903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ALOX12 antibody (483-663) (STJ116903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ALOX12 antibody (N-Term) (STJA0010318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ALOX12B Rabbit polyclonal antibody (STJ118650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALOX12B antibody (1-250) (STJ118650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using ALOX15 Rabbit polyclonal antibody (STJ28944) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15 antibody (1-230) (STJ28944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALOX15 antibody (156-206 aa) (STJ195947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ71782 (0. 2µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ALOX15 antibody (Internal) (STJ71782)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Immunofluorescence analysis of U2OS cells using ALOX15B Rabbit polyclonal antibody (STJ28945) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15B antibody (1-351) (STJ28945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALOX15B antibody (576-626 aa) (STJ195383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using ALOX5 antibody (STJ22592) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALOX5 antibody (1-200) (STJ22592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of U-2 OS cells using ALOX5 Rabbit monoclonal antibody (STJ11101834, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-ALOX5 antibody (100-200) [S4MR] (STJ11101834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Arachidonate 5 Lipoxygenase Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALOX5 antibody (246-295 aa) (STJ91392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ALOX5AP antibody (STJA0009801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using ALOXE3 Rabbit pAb (STJ110544) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ALOXE3 antibody (1-280) (STJ110544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ALOXE3 antibody (467-517) (STJA0006307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Alpaca-IgG antibody (STJA0010224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Alpaca

£48.00 - £252.00

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Anti-ALPG antibody (10-90 aa) (STJ190382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of AFP at dilution of 1:1000

Anti-alpha 1 Fetoprotein antibody [SM0476] (STJA0010476)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-alpha 1 Fetoprotein antibody [SM5089] (STJA0005089)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£170.50 - £233.50

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Anti-alpha A Crystallin antibody [PT1172R] (STJA0040211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot of mouse whole brain lysate showing specific immunolabeling of the ~105 kDa Alpha-actinin 4 protein.

Anti-Alpha Actinin 4 antibody [93] (STJA0003834)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse

£454.50

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Anti-Alpha Fetoprotein antibody (50-100) (STJ501651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

£427.50

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Anti-Alpha Fetoprotein antibody (50-100) {Biotin} (STJ501652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

£528.50

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Anti-Alpha Fetoprotein antibody (50-100) {FITC} (STJ501653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

£528.50

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Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin (STJA0003573, 1:5000, red) , labeling of the myelin sheaths around the axons with MBP (STJA0003695, 1:5000, green) , and additional nuclear staining with DAPI (blue).

Anti-Alpha Internexin/NF-66 antibody (STJA0003573)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£403.50

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Immunofluorescence of a section of rat cerebellum selectively labeling the axons of granule cells with alpha-internexin (STJA0003572, 1:5000, green) and colabeled with anti-calretinin (red).

Anti-Alpha Internexin/NF-66 antibody [1D2] (STJA0003572)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

£403.50

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Confocal imaging of paraffin-embedded Rat heart using alpha Sarcoglycan (SGCA) Rabbit monoclonal antibody (STJ11103115, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-alpha Sarcoglycan antibody (288-387) [S5MR] (STJ11103115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Anti-Alpha smooth muscle actin antibody [PT0074R] (STJA0039646)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-alpha smooth muscle Actin antibody [SM0388] (STJA0010388)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-alpha smooth muscle Actin antibody [SM0389] (STJA0010389)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunohistochemistry analysis of paraffin-embedded human kidney using Alpha-SMA at dilution of 1:500.

Anti-alpha smooth muscle Actin antibody [SM0514] (STJA0010514)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£233.50

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Western blot analysis of extracts of various cell lines, using Alpha SNAP antibody (STJ11102959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Alpha SNAP antibody (201-295) [S9MR] (STJ11102959)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-alpha Synuclein antibody (C-Term) (STJ13100579)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-Alpha Synuclein antibody (C-Term) (STJ13100584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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