• Western blot analysis of extracts of various cell lines, using ALAS1 antibody (STJ28604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ALAS1 antibody (1-250) (STJ28604)

SKU:
STJ28604

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALAS1 (1-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALAS1
Gene ID: 211
Uniprot ID: HEM1_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALAS1 (NP_954635.1).
Immunogen Sequence: MESVVRRCPFLSRVPQAFLQ KAGKSLLFYAQNCPKMMEVG AKPAPRALSTAAVHYQQIKE TPPASEKDKTAKAKVQQTPD GSQQSPDGTQLPSGHPLPAT SQGTASKCPFLAAQMNQRGS SVFCKASLELQEDVQEMNAV RKEVAETSAGPSVVSVKTDG GDPSGLLKNFQDIMQKQRPE RVSHLLQDNLPKSVSTFQYD RFFEKKIDEKKNDHTYRVFK TVNRRAHIFPMADDYSDSL
Post Translational Modifications In normoxia, is hydroxylated at Pro-576, promoting interaction with VHL, initiating ubiquitination and subsequent degradation via the proteasome. Ubiquitinated.in normoxia following hydroxylation and interaction with VHL, leading to its subsequent degradation via the proteasome.
Function Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products.
Protein Name 5-Aminolevulinate Synthase - Non-Specific - Mitochondrial
Alas-H
5-Aminolevulinic Acid Synthase 1
Delta-Ala Synthase 1
Delta-Aminolevulinate Synthase 1
Database Links Reactome: R-HSA-189451
Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Localizes To The Matrix Side Of The Mitochondrion Inner Membrane
Alternative Antibody Names Anti-5-Aminolevulinate Synthase - Non-Specific - Mitochondrial antibody
Anti-Alas-H antibody
Anti-5-Aminolevulinic Acid Synthase 1 antibody
Anti-Delta-Ala Synthase 1 antibody
Anti-Delta-Aminolevulinate Synthase 1 antibody
Anti-ALAS1 antibody
Anti-ALAS3 antibody
Anti-ALASH antibody
Anti-OK antibody
Anti-SW-cl.121 antibody

Information sourced from Uniprot.org

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