• STJ70624 (1µg/ml) staining of HepG2 (A) , Jurkat (B) , Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • HEK293 overexpressing AKT3 (RC224750) and probed with STJ70624 (mock transfection in second lane) , tested by Origene.

Anti-AKT3 antibody (Internal) (STJ70624)

SKU:
STJ70624-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AKT3 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IHC-5µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AKT3
Gene ID: 10000
Uniprot ID: AKT3_HUMAN
Immunogen Region: Internal
Accession Number: NP_005456.1; NP_859029.1
Specificity: This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1).
Immunogen Sequence: CSPTSQIDNIGEEEM
Post Translational Modifications Phosphorylation on Thr-305 and Ser-472 is required for full activity. Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.
Function AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Protein Name Rac-Gamma Serine/Threonine-Protein Kinase
Protein Kinase Akt-3
Protein Kinase B Gamma
Pkb Gamma
Rac-Pk-Gamma
Stk-2
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1257604
Reactome: R-HSA-1358803
Reactome: R-HSA-198323
Reactome: R-HSA-198693
Reactome: R-HSA-199418
Reactome: R-HSA-211163
Reactome: R-HSA-389357
Reactome: R-HSA-389513
Reactome: R-HSA-392451
Reactome: R-HSA-5218920
Reactome: R-HSA-5628897
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Reactome: R-HSA-6804758
Reactome: R-HSA-6804759
Reactome: R-HSA-69202
Reactome: R-HSA-69656
Reactome: R-HSA-8876198
Reactome: R-HSA-8941332
Reactome: R-HSA-8948751
Reactome: R-HSA-9607240
Reactome: R-HSA-9614399
Reactome: R-HSA-9634638
Reactome: R-HSA-9755511
Reactome: R-HSA-9755779
Cellular Localisation Nucleus
Cytoplasm
Membrane
Peripheral Membrane Protein
Membrane-Associated After Cell Stimulation Leading To Its Translocation
Alternative Antibody Names Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance