• Western blot analysis of various lysates using ALAS2 Rabbit polyclonal antibody (STJ28605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALAS2 antibody (50-320) (STJ28605)

SKU:
STJ28605

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALAS2 (50-320) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALAS2
Gene ID: 212
Uniprot ID: HEM0_HUMAN
Immunogen Region: 50-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human ALAS2 (NP_001033057.1).
Immunogen Sequence: VKTHQFLFGIGRCPILATQG PNCSQIHLKATKAGGDSPSW AKGHCPFMLSELQDGKSKIV QKAAPEVQEDVKAFKTGNYV FSYDQFFRDKIMEKKQDHTY RVFKTVNRWADAYPFAQHFS EASVASKDVSVWCSNDYLGM SRHPQVLQATQETLQRHGAG AGGTRNISGTSKFHVELEQE LAELHQKDSALLFSSCFVAN DSTLFTLAKILPGCEIYSDA GNHASMIQGIRNSGAAKFV
Tissue Specificity Isoform 1: Erythroid-specific. Isoform 2: Erythroid-specific. Isoform 3: Erythroid-specific. Isoform 4: Erythroid-specific.
Function Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. Contributes significantly to heme formation during erythropoiesis. Isoform 3: Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. Catalytic activity is 75-85% of isoform 1 activity. Isoform 4: Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. Catalytic activity is 65-75% of isoform 1 activity.
Protein Name 5-Aminolevulinate Synthase - Erythroid-Specific - Mitochondrial
Alas-E
5-Aminolevulinic Acid Synthase 2
Delta-Ala Synthase 2
Delta-Aminolevulinate Synthase 2
Database Links Reactome: R-HSA-189451
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Localizes To The Matrix Side Of The Mitochondrion Inner Membrane
Isoform 4: Mitochondrion Inner Membrane
Alternative Antibody Names Anti-5-Aminolevulinate Synthase - Erythroid-Specific - Mitochondrial antibody
Anti-Alas-E antibody
Anti-5-Aminolevulinic Acid Synthase 2 antibody
Anti-Delta-Ala Synthase 2 antibody
Anti-Delta-Aminolevulinate Synthase 2 antibody
Anti-ALAS2 antibody
Anti-ALASE antibody
Anti-ASB antibody

Information sourced from Uniprot.org

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