Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Acetyl-Histone H3-Lys56 antibody (STJ197305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H3 (Acetyl Lys9) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H3-Lys9 antibody (3-52 aa) (STJ90121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl Histone H3 K9 Mouse mAb diluted at 1:200.

Anti-Acetyl-Histone H3.1-K9 antibody [2E7] (STJ97749)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 Rabbit polyclonal antibody (STJ118031) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K12 antibody (STJ118031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K16 Rabbit polyclonal antibody (STJ11103217) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Acetyl-Histone H4-K16 antibody (STJ11103217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 (STJ116277) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. U2OS cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K16 antibody (STJ116277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ117427) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Acetyl-Histone H4-K5 antibody (1-13) (STJ117427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody (STJ11103295) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Acetyl-Histone H4-K5 antibody (STJ11103295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ11101677) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Acetyl-Histone H4-K5 antibody [S7MR] (STJ11101677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€183.13 - €803.13

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Anti-Acetyl-Histone H4-K5/K8/K12/K16 antibody [SRM] (STJ11107155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Chromatin immunoprecipitation was performed with 5 Mu g of cross-linked chromatin from HeLa, using 5 Mu g of Acetyl-Histone H4-K8 Rabbit polyclonal antibody (STJ29400) and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Anti-Acetyl-Histone H4-K8 antibody (STJ29400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 Rabbit polyclonal antibody (STJ11100937) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K91 antibody (STJ11100937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys12) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys12 antibody (10-59 aa) (STJ90128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys16 antibody (1-50 aa) (STJ90129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys5) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys5 antibody (1-50 aa) (STJ90126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys8) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys8 antibody (1-50 aa) (STJ90127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Anti-Acetyl-HMGA1-Lys15 antibody (STJA0005962)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGA1-Lys65 antibody (STJA0005963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGA1-Lys71 antibody (STJA0005964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit polyclonal antibody (STJ11102770) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-HMGB1-K29 antibody (STJ11102770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Acetyl-HMGB1-K30 antibody (STJ11106187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Acetyl-HMGB1-K90 antibody (STJ11106188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of HepG2 cells treated with UV using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HMGB1-Lys12 antibody (STJ97227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGB1-Lys3 antibody (STJA0005965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGB1-Lys82 antibody (STJA0005966)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGN1-Lys27/K33/K31 antibody (STJA0005967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGN2-Lys3 antibody (STJA0005969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HMGN2-Lys31 antibody (STJA0005968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of mouse-lung mouse-brain mouse-heart Hela mouse-liver lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HNF-4 Alpha/Gamma-Lys127/79 antibody (STJ98692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of mouse-lung mouse-kidney mouse-liver lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HNF4A-Lys106 antibody (106 aa) (STJ98691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of rat kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HSP70-Lys246 antibody (STJ97228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of K562 cells using Acetyl-HSP 90 (K292/284) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HSP90AA1-Lys292/284 antibody (251-300 aa) (STJ90144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of A549 cells using Acetyl-HSP 90 (K435) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HSP90AA1-Lys435 antibody (STJ90145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-HTT-Lys442 antibody (STJA0005970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of PC12 cells using Acetyl-AP-1 (K271) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-JUN-Lys271 antibody (STJ97229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of HepG2 cells using Acetyl-PCAF (K428) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-KAT2B-Lys428 antibody (STJ97360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Acetyl-Kine antibody (STJ197446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species independent

€60.00 - €315.00

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Anti-Acetyl-KLF1-Lys274 antibody (STJA0005972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Acetyl-KLF13-Lys166 antibody (STJA0005973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-Acetyl-KPNA2-Lys22 antibody (STJA0005974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of A549 cells using Acetyl-Cytokeratin 8 (K483) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-KRT8-Lys483 antibody (434-483 aa) (STJ90146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Acetyl-Lysine antibody (STJ90135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Plant

€60.00 - €315.00

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Western blot analysis of 1) Mouse Brain Tissue, 2) Hela, 3) Hela+TSA Treated using Acetyl Lysine monoclonal antibody.

Anti-Acetyl-Lysine antibody [10B10] (STJ97466)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Species independent

€60.00 - €315.00

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Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-MAPT-Lys174 antibody (174 aa) (STJ98828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of HELA 293T using Acetyl-Tau (K686) antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-MAPT-Lys686 antibody (STJ97701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-MEF2A-Lys3 antibody (STJA0005977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-MEF2A-Lys403 antibody (STJA0005976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-MYB-Lys471 antibody (STJA0005978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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