• Western blot analysis of HELA 293T using Acetyl-p300 (K1558/K1560) antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-EP300-Lys1560 antibody (STJ97697)


Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone acetyltransferase p300-Lys1560 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EP300
Gene ID: 2033
Uniprot ID: EP300_HUMAN
Specificity: Acetyl-p300 (K1558/K1560) Polyclonal Antibody detects endogenous levels of p300 around the acetylation site of K1558 protein.
Immunogen: Synthesized acetyl-peptide derived from the human p300 around the acetylation site of K1558.
Function Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of p53/TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Following DNA damage, forms a stress-responsive p53/TP53 coactivator complex with JMY which mediates p53/TP53 acetylation, thereby increasing p53/TP53-dependent transcription and apoptosis. Promotes chromatin acetylation in heat shock responsive HSP genes during the heat shock response (HSR), thereby stimulating HSR transcription. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acetylates 'Lys-247' of EGR2. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription.exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity. Acetylates XBP1 isoform 2.acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity. Acetylates PCNA.acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Acetylates MEF2D. Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degradation, this mechanism may be involved in CD4/CD8 lineage differentiation. Acetylates GABPB1, impairing GABPB1 heterotetramerization and activity. Acetylates PCK1 and promotes PCK1 anaplerotic activity. Acetylates RXRA and RXRG. Acetylates isoform M2 of PKM (PKM2), promoting its homodimerization and conversion into a protein kinase. Acetylates RPTOR in response to leucine, leading to activation of the mTORC1 complex. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA), 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA), lactoyl-CoA or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation, 2-hydroxyisobutyrylation, lactylation or propionylation, respectively. Acts as a histone crotonyltransferase.crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (2E)-butenoyl-CoA (crotonyl-CoA) concentration is low. Also acts as a histone butyryltransferase.butyrylation marks active promoters. Catalyzes histone lactylation in macrophages by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. Acts as a protein-lysine 2-hydroxyisobutyryltransferase.regulates glycolysis by mediating 2-hydroxyisobutyrylation of glycolytic enzymes. Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
Protein Name Histone Acetyltransferase P300
P300 Hat
E1a-Associated Protein P300
Histone Butyryltransferase P300
Histone Crotonyltransferase P300
Protein 2-Hydroxyisobutyryltransferase P300
Protein Lactyltransferas P300
Protein Propionyltransferase P300
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-1368082
Reactome: R-HSA-156711
Reactome: R-HSA-1912408
Reactome: R-HSA-1989781
Reactome: R-HSA-201722
Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2173796
Reactome: R-HSA-2197563
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3134973
Reactome: R-HSA-3214847
Reactome: R-HSA-3371568
Reactome: R-HSA-381340
Reactome: R-HSA-3899300
Reactome: R-HSA-400253
Reactome: R-HSA-5250924
Reactome: R-HSA-5617472
Reactome: R-HSA-5621575
Reactome: R-HSA-5689901
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804114
Reactome: R-HSA-6804758
Reactome: R-HSA-6804760
Reactome: R-HSA-6811555
Reactome: R-HSA-8866907
Reactome: R-HSA-8936459
Reactome: R-HSA-8939243
Reactome: R-HSA-8941856
Reactome: R-HSA-8941858
Reactome: R-HSA-8951936
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Reactome: R-HSA-9018519
Reactome: R-HSA-9029569
Reactome: R-HSA-9031628
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-9614657
Reactome: R-HSA-9616222
Reactome: R-HSA-9617629
Reactome: R-HSA-9701898
Reactome: R-HSA-9707616
Reactome: R-HSA-9735871
Reactome: R-HSA-9759194
Reactome: R-HSA-9793380
Reactome: R-HSA-9818027
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Localizes To Active Chromatin: Colocalizes With Histone H3 Acetylated And/Or Crotonylated At 'Lys-18' (H3k18ac And H3k18cr
In The Presence Of Alx1 Relocalizes From The Cytoplasm To The Nucleus
Colocalizes With Rock2 In The Nucleus
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Histone Acetyltransferase P300 antibody
Anti-P300 Hat antibody
Anti-E1a-Associated Protein P300 antibody
Anti-Histone Butyryltransferase P300 antibody
Anti-Histone Crotonyltransferase P300 antibody
Anti-Protein 2-Hydroxyisobutyryltransferase P300 antibody
Anti-Protein Lactyltransferas P300 antibody
Anti-Protein Propionyltransferase P300 antibody
Anti-EP300 antibody
Anti-P300 antibody

Information sourced from Uniprot.org

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