Anti-Acetyl-E2F4-Lys96 antibody (61-110 aa) (STJ90140)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized Acetyl-peptide derived from the human E2F4 around the Acetylation site of Lys96 at the amino acid range 61-110
General Information
| Short Description | Rabbit polyclonal anti-Acetyl-Transcription factor E2F4-Lys96 (61-110 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | E2F4 |
| Gene ID | 1874 |
| Uniprot ID | E2F4_HUMAN |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from the human E2F4 around the Acetylation site of Lys96 at the amino acid range 61-110 |
| Immunogen Region | 61-110 aa |
| Specificity | Acetyl-E2F-4 (K96) Polyclonal Antibody detects endogenous levels of E2F-4 protein only when acetylated at K96. |
Additional Info
| Post Translational Modifications | Differentially phosphorylated in vivo. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis. |
| Protein Name | Transcription Factor E2f4E2f-4 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-2173796Reactome: R-HSA-6804114Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f4 antibodyAnti-E2f-4 antibodyAnti-E2F4 antibody |
Information sourced from Uniprot.org