Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ERBB2 antibody [11H9] (STJ96947)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Neu monoclonal antibody against truncated Neu recombinant protein.

Anti-ERBB2 antibody [9B9D8] (STJ98270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of ERBB2 at dilution of 1:1000

Anti-ErbB2 antibody [SM0478] (STJA0010478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of 293T cells using ErbB-3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERBB3 antibody (1-50 aa) (STJ92976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using ErbB-3 monoclonal antibody against truncated Trx-ErbB-3 recombinant protein (1) and MBP-ErbB-3 (aa1175-1275) recombinant protein (2).

Anti-ERBB3 antibody (1175-1275 aa) [3F10F6] (STJ98042)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using ErbB-3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERBB3 antibody (1191-1240 aa) (STJ92975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from normal (control) and ErbB3/HER3 knockout (KO) 293T cells, using ErbB3/HER3 antibody (STJ27527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ERBB3 antibody (1275-1342) (STJ27527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-ERBB3 antibody (1293-1342 aa) (STJ92973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using ErbB3/HER3 Rabbit polyclonal antibody (STJ11103843) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ERBB3 antibody (20-285) (STJ11103843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using ErbB-3 monoclonal antibody against human ERBB3 (aa22-369) -hIgGFc trasfected HEK293 cell lysate (1).

Anti-ERBB3 antibody (22-369 aa) [2F9] (STJ98044)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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STJ70764 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ERBB3/HER3 antibody (C-Term) (STJ70764)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Immunofluorescence analysis of U-2 OS cells using ErbB4/HER4 Rabbit polyclonal antibody (STJ27886) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ERBB4 antibody (1150-1250) (STJ27886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells, using HER4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ERBB4 antibody (1250-1299 aa) (STJ92978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-ERCC1 (PT0756) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2Predicted band size: 33kDaObserved band size: 33kDa

Anti-ERCC1 antibody (1-100 aa) (STJ196164)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of 3T3 cells using ERCC1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERCC1 antibody (141-190 aa) (STJ92979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ73597 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ERCC1 antibody (Internal) (STJ73597)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of 1) Hela, 2) HepG2, 3) 293T, 4) Jurkat, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERCC1 antibody [1B10] (STJ96980)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Anti-ERCC1 antibody [SM4986] (STJA0004986)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunofluorescence analysis of HeLa cells using XPD/XPD/ERCC2 antibody (STJ27607). Blue: DAPI for nuclear staining.

Anti-ERCC2 antibody (1-300) (STJ27607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using ERCC5 Polyclonal Antibody (STJ118490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ERCC5 antibody (1-270) (STJ118490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using XPG Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERCC5 antibody (131-180 aa) (STJ96280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ERCC6 antibody (141-190 aa) (STJ92493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver, antibody was diluted at 1:100

Anti-EREG antibody (71-120 aa) (STJ99008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using ERF Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERF antibody (492-541 aa) (STJ92983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human kidney using ERG antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ERG antibody (400-479 aa) [R09-9J7] (STJA0014548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/ChIP

Reactivity :

Human/Mouse/Rat

£264.50

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STJ70848 (0. 1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ERG antibody (C-Term) (STJ70848)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC/IF

Reactivity :

Human

£312.50

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Immunofluorescence analysis of HepG2 cells, using ERGI3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ERGIC3 antibody (321-370 aa) (STJ92985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of human-lung tissue. 1, ERK 1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-ERK 1/2 antibody (330-379 aa) (STJ92990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using ERK1/2 Mouse mAb diluted at 1:200.

Anti-ERK1/2 antibody (140-220 aa) [1H4] (STJ97783)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse/Fish

£48.00 - £252.00

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Western blot analysis using ERK 1/2 monoclonal antibody against Jurkat (1) , HeLa (2) , A431 (3) and NIH/3T3 (4) cell lysate.

Anti-ERK1/2 antibody [3F8] (STJ98046)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain Tissue with P44/42 MAPK (ERK1/2) Mouse mAb diluted at 1:2, 000.

Anti-ERK1/2 antibody [6B1] (STJ97534)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70635 (0. 5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ERK2/MAPK1 antibody (Internal) (STJ70635)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis using IRE1 Alpha monoclonal antibody against Raji (1) , A431 (2) , Jurkat (3) , HeLa (4) and HEK293 (5) cell lysate.

Anti-ERN1 antibody (282-433 aa) [9F2] (STJ98183)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of PC-12 cells using IRE1 Rabbit polyclonal antibody (STJ119924) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ERN1 antibody (286-435) (STJ119924)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells, using ERN2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ERN2 antibody (841-890 aa) (STJ93759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ERp29 Rabbit pAb (STJ110266) at 1:1000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 1s.

Anti-ERP29 antibody (33-261) (STJ110266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ71752 (0. 1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ERP29 antibody (C-Term) (STJ71752)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Rat/Dog/Pig/Cow

£312.50

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Anti-Erythroid Marker antibody [SFL23.6] (STJ16100764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Western blot analysis of various cells using Separase Polyclonal Antibody diluted at 1：1000

Anti-ESPL1 antibody (767-816 aa) (STJ95608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 using ESR Alpha Rabbit monoclonal antibody (STJ11104746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ESR1 antibody (100-200) [S4746RM] (STJ11104746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using ER Alpha Polyclonal Antibody diluted at 1：500

Anti-ESR1 antibody (136-185 aa) (STJ92997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ESR1 antibody (501-550 aa) (STJ92999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ER Alpha monoclonal antibody against MCF-7 (1) , T47D (2) and SKBR3 (3) cell lysate.

Anti-ESR1 antibody [6B6] (STJ98054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-ER-β (PT0318) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HEK293 Lane 3: MCF7Predicted band size: 59kDaObserved band size: 59kDa

Anti-ESR2 antibody (400-530 aa) (STJ196169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of mouse-kidney tissue. 1, ER Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-ESR2 antibody (71-120 aa) (STJ93002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ESRRA antibody (STJ116117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ESRRA antibody (1-76) (STJ116117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ESRRA antibody (STJ23577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ESRRA antibody (1-76) (STJ23577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Estrogen Receptor alpha antibody [SM0443] (STJA0010443)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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