• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Collagen alpha1 XVIII Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL18A1 antibody (1301-1350 aa) (STJ92380)

SKU:
STJ92380

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Collagen alpha-1XVIII chain (1301-1350 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL18A1
Gene ID: 80781
Uniprot ID: COIA1_HUMAN
Immunogen Region: 1301-1350 aa
Specificity: COL18A1 Polyclonal Antibody detects endogenous levels of COL18A1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Collagen alpha1 XVIII at the amino acid range 1301-1350
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated. Circulating endostatins are found as sialoglycoprotein and asialoglycoprotein structures. Undergoes proteolytic processing by CTSL/cathepsin-L and elastase-like proteases to generate both non-collagenous domain 1 trimers and endostatin monomers. In tissue extracts (brain, skeletal muscle, heart, kidney, testis and liver) predominantly bands of approximately 38 kDa are detected.recombinant non-collagenous domain 1 shows similar mobility. In vitro, several proteolytic cleavage sites in the non-collagenous domain 1 hinge region generating different endostatin-like peptides are reported.
Function Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Non-collagenous domain 1: May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells.the function requires homotrimerization and implicates MAPK signaling. Endostatin: Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5. May negatively regulate the activity of homotrimeric non-collagenous domain 1.
Protein Name Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1
Nc1
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1592389
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000157
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Non-Collagenous Domain 1: Secreted
Endostatin: Secreted
Alternative Antibody Names Anti-Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1 antibody
Anti-Nc1 antibody
Anti-COL18A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance