Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of extracts of HeLa cells, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) mouse monoclonal antibody (STJ119899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (19-609) [S9MM] (STJ119899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various lysates, using Alpha-Fetoprotein (AFP) Rabbit polyclonal antibody (STJ22541) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (360-609) (STJ22541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of mouse liver, mouse serum cells using AFP Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-AFP antibody (371-420 aa) (STJ96505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) Rabbit polyclonal antibody (red, A17457) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AFP antibody (450-550) (STJ113691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, AFP alpha 1 Fetoprotein monoclonal antibody (17C5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-AFP antibody [17C5] (STJ96977)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$64.80 - $340.20

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Anti-AFP antibody [C2] (STJ16100499)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF/IHC

Reactivity :

Human/Mouse

$388.13 - $1,788.08

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Anti-AFP antibody [C3] (STJ16100500)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF/IHC

Reactivity :

Dog/Human/Monkey/Pig

$388.13 - $1,788.08

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Anti-AFP antibody [D10] (STJ16100501)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF/IHC/RIM

Reactivity :

Human

$388.13 - $1,788.08

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Anti-AFP antibody [MBS-12] (STJ16100498)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF/IHC

Reactivity :

Human

$388.13 - $1,788.08

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Alpha-Fetoprotein (AFP) Mouse monoclonal antibody (STJ113435) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AFP antibody [S5MM] (STJ113435)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of various cells using CCP2 Polyclonal Antibody diluted at 1：2000

Anti-AGBL2 antibody (731-780 aa) (STJ92074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CBCP3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AGBL3 antibody (611-660 aa) (STJ92075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of 3T3 cells using RAGE Polyclonal Antibody diluted at 1：1000

Anti-AGER antibody (133-182 aa) (STJ95358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates, using AGMAT Rabbit polyclonal antibody (STJ114084) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-AGMAT antibody (1-352) (STJ114084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Immunocytochemistry analysis of Argonaute 2 (green) in HCT116 using Argonaute 2 antibody, and DAPI (blue).

Anti-AGO2 antibody (381-396 aa) [R04-5H0] (STJA0010979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$357.08

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Immunofluorescence analysis of L929 cells using Argonaute-2 Polyclonal Antibody (STJ29944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AGO2 antibody (47-115) (STJ29944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using AGPAT2 antibody (STJ28601). Blue: DAPI for nuclear staining.

Anti-AGPAT2 antibody (199-278) (STJ28601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using AGPS antibody (STJ112509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AGPS antibody (379-658) (STJ112509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Confocal imaging of NIH/3T3 cells using AGR2 Rabbit monoclonal antibody (STJ11101412, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody ( dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-AGR2 antibody (1-100) [S2MR] (STJ11101412)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Immunofluorescence analysis of U-2 OS cells using AGR2 antibody (STJ29144) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AGR2 antibody (21-175) (STJ29144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of COS7 cells, using AGR3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AGR3 antibody (1-50 aa) (STJ91507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of NIH/3T3 cells using AGRN Rabbit polyclonal antibody (STJ119450) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AGRN antibody (30-350) (STJ119450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using Angiotensinogen antibody (STJ118084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AGT antibody (35-485) (STJ118084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of HEPG2 cells using AT1 Polyclonal Antibody diluted at 1：500

Anti-AGTR1 antibody (101-150 aa) (STJ91737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using AGTRAP antibody (STJ114071) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AGTRAP antibody (93-152) (STJ114071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ71786 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-AGXT/AGT antibody (Internal) (STJ71786)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

$421.88

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Western blot analysis of various cells using Ah Receptor Polyclonal Antibody

Anti-Ah Receptor antibody (2-51 aa) (STJ91510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using AHCY antibody (STJ27253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AHCY antibody (253-432) (STJ27253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of A-549 cells using AHNAK Rabbit polyclonal antibody (STJ11103983) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AHNAK antibody (1-149) (STJ11103983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-AHNAK antibody (5720-5800 aa) (STJ190105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AHR antibody (408-458 aa) (STJ193903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Fish

$64.80 - $340.20

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Anti-AHR antibody (619-848 aa) [SRM] (STJ11106859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Western blot analysis of extracts of various cell lines, using AHR antibody (STJ22550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-AHR antibody (619-848) (STJ22550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ71870 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-AHR antibody (Internal) (STJ71870)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human

$421.88

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Western blot analysis of various cells using AHA-1 Polyclonal Antibody diluted at 1：1000

Anti-AHSA1 antibody (161-210 aa) (STJ91511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-AHSP antibody (1-51 aa) (STJ196177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

$64.80 - $340.20

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STJ70153 staining (0. 5µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-AIBZIP/CREB3L4 antibody (C-Term) (STJ70153)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

$421.88

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Western blot analysis of Hela, HepG2 cells using AID Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-AICDA antibody (81-130 aa) (STJ97261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of paraffin-embedded rat brain using AIF1/IBA1 Rabbit polyclonal antibody (STJ11100899) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AIF1 antibody (1-147) (STJ11100899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded rat brain using AIF1/IBA1 Rabbit polyclonal antibody (STJ114929) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AIF1 antibody (1-147) (STJ114929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AIF1 antibody (STJ192269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AIF1/IBA1 antibody (1-100) [S7MR] (STJ11103127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-AIF1/IBA1 antibody (1-147 aa) [SRM] (STJ11106866)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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STJ70928 (4µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Anti-AIF1/IBA1-Isoform 1/3 antibody (C-Term) (STJ70928)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human

$421.88

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STJ70888 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-AIF1/IBA1-Isoform 3 antibody (Internal) (STJ70888)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse/Dog

$421.88

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STJ73111 (0. 1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIFM1 antibody (183-195) (STJ73111)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Cow

$421.88

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Immunofluorescence analysis of C6 cells using AIF antibody (STJ11103814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AIFM1 antibody (334-613) (STJ11103814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Confocal immunofluorescence analysis of HeLa cells using [KO Validated] AIF Rabbit polyclonal antibody (STJ22557) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-AIFM1 antibody (334-613) (STJ22557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options