• Western blot analysis of extracts of various cell lines, using ADAR11 rabbit polyclonal antibody (STJ110179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HepG2 cells using ADAR1 Rabbit polyclonal antibody (STJ110179) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat spleen using ADAR11 antibody (STJ110179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using ADAR1 Rabbit polyclonal antibody (STJ110179) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using ADAR11 antibody (STJ110179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of 293F, using ADAR1 antibody (STJ110179) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded mouse spleen using ADAR11 antibody (STJ110179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ADAR antibody (212-228) (STJ110179)

SKU:
STJ110179

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAR (212-228) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAR
Gene ID: 103
Uniprot ID: DSRAD_HUMAN
Immunogen Region: 212-228
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 212-228 of human ADAR11 (NP_001102.2).
Immunogen Sequence: RPDGHSQGAPNSDPSLE
Tissue Specificity Ubiquitously expressed, highest levels were found in brain and lung. Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.
Post Translational Modifications Sumoylation reduces RNA-editing activity.
Function Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins since the translational machinery read the inosine as a guanosine.pre-mRNA splicing by altering splice site recognition sequences.RNA stability by changing sequences involved in nuclease recognition.genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication.and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication.
Protein Name Double-Stranded Rna-Specific Adenosine Deaminase
Drada
136 Kda Double-Stranded Rna-Binding Protein
P136
Interferon-Inducible Protein 4
Ifi-4
K88dsrbp
Database Links Reactome: R-HSA-75102
Reactome: R-HSA-77042
Reactome: R-HSA-909733
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Shuttles Between The Cytoplasm And Nucleus
Nuclear Import Is Mediated By Tnpo1
Isoform 5: Cytoplasm
Nucleolus
Predominantly Nuclear But Can Shuttle Between Nucleus And Cytoplasm
Tnpo1 Can Mediate Its Nuclear Import Whereas Xpo5 Can Mediate Its Nuclear Export
Alternative Antibody Names Anti-Double-Stranded Rna-Specific Adenosine Deaminase antibody
Anti-Drada antibody
Anti-136 Kda Double-Stranded Rna-Binding Protein antibody
Anti-P136 antibody
Anti-Interferon-Inducible Protein 4 antibody
Anti-Ifi-4 antibody
Anti-K88dsrbp antibody
Anti-ADAR antibody
Anti-ADAR1 antibody
Anti-DSRAD antibody
Anti-G1P1 antibody
Anti-IFI4 antibody

Information sourced from Uniprot.org

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