Immunocytochemistry Antibodies | St John's Laboratory

Anti-Phospho-MAPK1 antibody [M206] (STJA0005411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$538.89

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Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (STJA0003631, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.

Anti-Phospho-MAPK1/2-Thr202/Tyr204 antibody (STJA0003631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Xenopus/Zebrafish

$674.13

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (STJ110835) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody (STJ110835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (STJ11102568) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (STJ11102154) at1:3000 dilution (lower) incubated overnight at 4 °C. 293T cells were treated by PMA (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 10s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody [S8MR] (STJ11102568)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (STJ110862) at 1:18000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-MAPK14-T180/Y182 antibody (STJ110862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-Mapk14-Thr-180/Tyr-182 antibody [M139] (STJA0005505)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$544.41

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Anti-Phospho-Mapk14-Tyr-323 antibody (STJA0005506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$507.15

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Western blot analysis of various lysates using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (STJ22261) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-T202 antibody (STJ22261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 + ERK2-Y187 Rabbit pAb (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-MAPK8-Thr-183/Tyr-185 antibody [M268] (STJA0005456)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$504.39

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Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (STJ113512) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK8/MAPK9/MAPK10-T183/T183/T221 antibody [S2MR] (STJ113512)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S262 Rabbit pAb (STJ22274) at 1:500 dilution. Mouse brain were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 120s.

Anti-Phospho-MAPT-S262 antibody (STJ22274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from SH-SY5Y and C6 cells, using Phospho-Tau-S404 Rabbit pAb (STJ22272) at 1:2000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100nM) for 1 hour and Calyculin A (100nM) for 30 minutes after serum-starvation overnight. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-MAPT-S404 antibody (STJ22272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunofluorescence analysis of paraffin-embedded Rat brain using Phospho-Tau-Ser404 Rabbit pAb (STJ11101134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-MAPT-Ser404 antibody (STJ11101134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Tau-Ser416 Rabbit pAb (STJ24499). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAPT-Ser416 antibody (STJ11101135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-Tau-T231 Rabbit mAb (STJ11102551, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Phospho-MAPT-T231 antibody [S1MR] (STJ11102551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from HeLa cells, using Phospho-Tau-T181 Rabbit pAb (STJ11101032) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-MAPT-T498 antibody (STJ11101032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-MCM2-Ser108 antibody [R01-7G9] (STJA0034547)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-MCM2-Ser27 antibody [R08-4D8] (STJA0035076)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-MCM2-Ser27 antibody [R88-9Q-1] (STJA0035685)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-MECP2-Ser-421 antibody (STJA0005465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$504.39

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Anti-Phospho-MeCP2-Ser-421 antibody (STJA0041282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-MECP2-Ser-80 antibody (STJA0005464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

$504.39

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Anti-Phospho-MeCP2-Ser-80 antibody (STJA0041281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-MEK1-Ser298 antibody [R07-1I6] (STJA0034555)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot with Anti-Phospho-MERTK Antibody (STJ502558) on mouse macrophage. Antibody at 1:800 dilution.

Anti-Phospho-MERTK antibody (746-757) (STJ502558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/FC/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$686.55

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Anti-Phospho-MERTK antibody (746-757) {Biotin} (STJ502559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/FC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$761.07

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Anti-Phospho-MERTK antibody (746-757) {FITC} (STJ502560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/FC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$761.07

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Anti-Phospho-MKK3+MKK6-S189+S207 antibody [SRM13703] (STJ11108433)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$0.00

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Anti-Phospho-MSK1-Ser360 antibody [R03-1G8] (STJA0033901)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

$184.23 - $388.47

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Anti-Phospho-MSK1-Ser376 antibody [R84-3U-1] (STJA0035666)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Western blot analysis of lysates from C6 cells, using Phospho-mTOR-S2448 Rabbit pAb (STJ27512) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-mTOR-S2448 antibody (STJ27512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-Myb-Ser11 antibody [R11-7D-7] (STJA0035662)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-Myc-Ser62 antibody [R09-1E2] (STJA0035954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-Myc-Ser62 antibody [R09-3K1] (STJA0034184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-Myc-Thr58 antibody [R09-1M8] (STJA0036004)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-MYH9-S1943 antibody (STJA0005473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$507.15

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Anti-Phospho-MYL12A-Ser-1 antibody (STJA0005479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Bovine

$507.15

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Anti-Phospho-MYL12A-Ser-19 antibody (STJA0005480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$507.15

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Anti-Phospho-Myosin IIA Heavy Chain, phospho-specific-S1943 antibody (STJA0041273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Anti-Phospho-Myosin Light Chain-Ser-1 antibody (STJA0041270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Bovine/Human/Mouse/Rat

$748.65

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Anti-Phospho-Myosin Light Chain-Ser-19 antibody (STJA0041275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

$748.65

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Anti-Phospho-N-Cadherin-Tyr-820 antibody (STJA0041143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-N-Cadherin-Tyr-860 antibody (STJA0041147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-N-WASP-Ser-484/Ser-485 antibody (STJA0041453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-N-WASP-Tyr-256 antibody (STJA0041455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-N/A-p-Tyr antibody (STJA0005529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

$440.91

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Anti-Phospho-N/A-p-Tyr antibody [M375] (STJA0005528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

$440.91

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